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PMID: 12451137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Glia induce dendritic growth in cultured sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 22 ·No. 23 ·2002-12-01 ·Pages 10377-87

Lein PJ, Beck HN, Chandrasekaran V, Gallagher PJ, Chen HL, Lin Y, Guo X, Kaplan PL, Tiedge H, Higgins D

Abstract

Dendritic growth in cultured sympathetic neurons requires specific trophic interactions. Previous studies have demonstrated that either coculture with glia or exposure to recombinant bone morphogenetic proteins (BMPs) is both necessary and sufficient to induce dendrite formation. These observations led us to test the hypothesis that BMPs mediate glial-induced dendritic growth. In situ hybridization and immunocytochemical studies indicate that the spatiotemporal expression of BMP5, -6, and -7 in rat superior cervical ganglia (SCG) is consistent with their proposed role in dendritogenesis. In vitro, both SCG glia and neurons were found to express BMP mRNA and protein when grown in the presence or absence of the other cell type. However, addition of ganglionic glia to cultured sympathetic neurons causes a marked increase in BMP proteins coincident with a significant decrease in follistatin and noggin. Functional assays indicate that glial-induced dendritic growth is significantly reduced by BMP7 antibodies and completely inhibited by exogenous noggin and follistatin. These data suggest that glia influence the rapid perinatal expansion of the dendritic arbor in sympathetic neurons by increasing BMP activity via modulation of the balance between BMPs and their antagonists.

MeSH Terms
Active Transport, Cell Nucleus/physiology Animals Antibodies/pharmacology Bone Morphogenetic Proteins/antagonists & inhibitors,genetics,metabolism Carrier Proteins Cell Division/physiology Cells, Cultured Coculture Techniques DNA-Binding Proteins/metabolism Dendrites/drug effects,physiology Down-Regulation/physiology Follistatin/genetics,metabolism Humans In Situ Hybridization Neuroglia/cytology,drug effects,physiology Neurons/cytology,drug effects,metabolism Proteins/genetics,metabolism RNA, Messenger/metabolism Rats Rats, Sprague-Dawley Signal Transduction/drug effects,physiology Smad Proteins Sympathetic Nervous System/cytology,drug effects,metabolism Trans-Activators/metabolism Up-Regulation/physiology
Chemicals
Antibodies Bone Morphogenetic Proteins Carrier Proteins DNA-Binding Proteins Follistatin Proteins RNA, Messenger Smad Proteins Trans-Activators noggin protein
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Lein Pamela J
Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205-2179, USA. plein@jhsph.edu
Beck Hiroko Nagasawa
Chandrasekaran Vidya
Gallagher Patrick J
Chen Hui-Ling
Lin Yuan
Guo Xin
Kaplan Paul L
Tiedge Henri
Higgins Dennis
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Email
plein@jhsph.edu
Published
2002-12-01
Pages
10377-87
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6758753
Subset
IM
Grants
NIEHS NIH HHS · P30 ES003819 · United States
NINDS NIH HHS · 1 R15 NS/OD36401-01 · United States
NIEHS NIH HHS · 2 P30 ES03819-14 · United States
NINDS NIH HHS · R01 NS34158 · United States
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PubMed source PMC Full Text View journal details
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