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PMID: 12368499 Published · ppublish English Journal Article

Expression profile analysis of the low-oxygen response in Arabidopsis root cultures.

The Plant cell ·Vol. 14 ·No. 10 ·2002-10-00 ·Pages 2481-94

Klok EJ, Wilson IW, Wilson D, Chapman SC, Ewing RM, Somerville SC, Peacock WJ, Dolferus R, Dennis ES

Abstract

We used DNA microarray technology to identify genes involved in the low-oxygen response of Arabidopsis root cultures. A microarray containing 3500 cDNA clones was screened with cDNA samples taken at various times (0.5, 2, 4, and 20 h) after transfer to low-oxygen conditions. A package of statistical tools identified 210 differentially expressed genes over the four time points. Principal component analysis showed the 0.5-h response to contain a substantially different set of genes from those regulated differentially at the other three time points. The differentially expressed genes included the known anaerobic proteins as well as transcription factors, signal transduction components, and genes that encode enzymes of pathways not known previously to be involved in low-oxygen metabolism. We found that the regulatory regions of genes with a similar expression profile contained similar sequence motifs, suggesting the coordinated transcriptional control of groups of genes by common sets of regulatory factors.

MeSH Terms
Arabidopsis/drug effects,genetics Culture Techniques DNA, Complementary/genetics Gene Expression Profiling/methods Gene Expression Regulation, Plant/drug effects Multigene Family/genetics Oligonucleotide Array Sequence Analysis Oxygen/pharmacology Plant Roots/drug effects,genetics Signal Transduction/genetics Time Factors
Chemicals
DNA, Complementary Oxygen
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Klok Erik Jan
Commonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, Australian Capital Territory 2601, Australia. erikjan.klok@csiro.au
Wilson Iain W
Wilson Dale
Chapman Scott C
Ewing Rob M
Somerville Shauna C
Peacock W James
Dolferus Rudy
Dennis Elizabeth S
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.004747
Email
erikjan.klok@csiro.au
Published
2002-10-00
Pages
2481-94
Language
English
Region
England
NLM ID
9208688
PMCID
PMC151230
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.004747 PMC Full Text View journal details
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