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PMID: 12271122 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 20 ·2002-10-01 ·Pages 13148-53

Paulsen IT, Seshadri R, Nelson KE, Eisen JA, Heidelberg JF, Read TD, Dodson RJ, Umayam L, Brinkac LM, Beanan MJ, Daugherty SC, Deboy RT, Durkin AS, Kolonay JF, Madupu R, Nelson WC, Ayodeji B, Kraul M, Shetty J, Malek J, Van Aken SE, Riedmuller S, Tettelin H, Gill SR, White O, Salzberg SL, Hoover DL, Lindler LE, Halling SM, Boyle SM, Fraser CM

Abstract

The 3.31-Mb genome sequence of the intracellular pathogen and potential bioterrorism agent, Brucella suis, was determined. Comparison of B. suis with Brucella melitensis has defined a finite set of differences that could be responsible for the differences in virulence and host preference between these organisms, and indicates that phage have played a significant role in their divergence. Analysis of the B. suis genome reveals transport and metabolic capabilities akin to soil/plant-associated bacteria. Extensive gene synteny between B. suis chromosome 1 and the genome of the plant symbiont Mesorhizobium loti emphasizes the similarity between this animal pathogen and plant pathogens and symbionts. A limited repertoire of genes homologous to known bacterial virulence factors were identified.

MeSH Terms
Alphaproteobacteria/genetics Brucella/genetics,pathogenicity Brucella melitensis/genetics Chromosomes, Bacterial/ultrastructure DNA Transposable Elements Genome, Bacterial Models, Genetic Molecular Sequence Data Open Reading Frames Rhizobium/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
31 authors, click to expand affiliations / ORCID
Paulsen Ian T
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. ipaulsen@tigr.org
Seshadri Rekha
Nelson Karen E
Eisen Jonathan A
Heidelberg John F
Read Timothy D
Dodson Robert J
Umayam Lowell
Brinkac Lauren M
Beanan Maureen J
Daugherty Sean C
Deboy Robert T
Durkin A Scott
Kolonay James F
Madupu Ramana
Nelson William C
Ayodeji Bola
Kraul Margaret
Shetty Jyoti
Malek Joel
Van Aken Susan E
Riedmuller Steven
Tettelin Herve
Gill Steven R
White Owen
Salzberg Steven L
Hoover David L
Lindler Luther E
Halling Shirley M
Boyle Stephen M
Fraser Claire M
References (45)
45 references, click to expand
  1. The genome sequence of the facultative intracellular pathogen Brucella melitensis.
    Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):443-8 PMID: 11756688
  2. Potential biological weapons threats.
    Emerg Infect Dis. 1999 Jul-Aug;5(4):523-7 PMID: 10458957
  3. Recommendations for the description of species and biotypes of the genus Brucella.
    Dev Biol Stand. 1976;31:27-37 PMID: 1261743
  4. Pathogenesis of placentitis in the goat inoculated with Brucella abortus. II. Ultrastructural studies.
    Vet Pathol. 1986 May;23(3):227-39 PMID: 3088810
  5. Identification of fibronectins in peritoneal macrophages during the phagocytosis of Brucella. An immunocytochemical study by electron microscopy.
    Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;52(2):169-76 PMID: 2878532
  6. Cloning and expression of an adhesin (AIDA-I) involved in diffuse adherence of enteropathogenic Escherichia coli.
    Infect Immun. 1989 May;57(5):1506-11 PMID: 2565291
  7. Pathogenesis of Brucella.
    Crit Rev Microbiol. 1990;17(3):209-30 PMID: 2154232
  8. Vaccination against Brucella.
    Adv Biotechnol Processes. 1990;13:147-68 PMID: 2185782
  9. Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria.
    J Bacteriol. 1990 Jul;172(7):3569-76 PMID: 2113907
  10. Macrophages and Brucella.
    Immunol Ser. 1994;60:363-80 PMID: 8251581
  11. Characterization of a two-gene locus from Bartonella bacilliformis associated with the ability to invade human erythrocytes.
    Infect Immun. 1995 Apr;63(4):1552-62 PMID: 7890422
  12. TopPred II: an improved software for membrane protein structure predictions.
    Comput Appl Biosci. 1994 Dec;10(6):685-6 PMID: 7704669
  13. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
    Science. 1995 Jul 28;269(5223):496-512 PMID: 7542800
  14. An overview of human brucellosis.
    Clin Infect Dis. 1995 Aug;21(2):283-9; quiz 290 PMID: 8562733
  15. Purification, characterization, and genetic analysis of Mycobacterium tuberculosis urease, a potentially critical determinant of host-pathogen interaction.
    J Bacteriol. 1995 Oct;177(19):5644-52 PMID: 7559354
  16. Asymmetric substitution patterns in the two DNA strands of bacteria.
    Mol Biol Evol. 1996 May;13(5):660-5 PMID: 8676740
  17. Salmonella enterica serotype Typhimurium ShdA is an outer membrane fibronectin-binding protein that is expressed in the intestine.
    Mol Microbiol. 2002 Feb;43(4):895-905 PMID: 11929540
  18. Flexible sequence similarity searching with the FASTA3 program package.
    Methods Mol Biol. 2000;132:185-219 PMID: 10547837
  19. Improved microbial gene identification with GLIMMER.
    Nucleic Acids Res. 1999 Dec 1;27(23):4636-41 PMID: 10556321
  20. The Pfam protein families database.
    Nucleic Acids Res. 2000 Jan 1;28(1):263-6 PMID: 10592242
  21. Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis.
    Infect Immun. 2000 Mar;68(3):1297-303 PMID: 10678941
  22. Agriculture and food security.
    Ann N Y Acad Sci. 1999;894:9-17 PMID: 10681963
  23. Foreign animal disease agents as weapons in biological warfare.
    Ann N Y Acad Sci. 1999;894:100-4 PMID: 10681977
  24. Intracellular trafficking of Brucella abortus in J774 macrophages.
    Infect Immun. 2000 Jul;68(7):4255-63 PMID: 10858243
  25. Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes.
    J Mol Biol. 2000 Aug 4;301(1):75-100 PMID: 10926494
  26. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
    Nature. 2000 Aug 3;406(6795):477-83 PMID: 10952301
  27. Hemin-binding surface protein from Bartonella quintana.
    Infect Immun. 2000 Dec;68(12):6750-7 PMID: 11083791
  28. Brucella suis-impaired specific recognition of phagosomes by lysosomes due to phagosomal membrane modifications.
    Infect Immun. 2001 Jan;69(1):486-93 PMID: 11119541
  29. TIGRFAMs: a protein family resource for the functional identification of proteins.
    Nucleic Acids Res. 2001 Jan 1;29(1):41-3 PMID: 11125044
  30. Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity.
    J Bacteriol. 2001 Jan;183(2):426-34 PMID: 11133934
  31. Limited genetic diversity of Brucella spp.
    J Clin Microbiol. 2001 Jan;39(1):235-40 PMID: 11136777
  32. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.
    Nature. 2001 Jan 25;409(6819):529-33 PMID: 11206551
  33. Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti.
    DNA Res. 2000 Dec 31;7(6):331-8 PMID: 11214968
  34. Evidence for symmetric chromosomal inversions around the replication origin in bacteria.
    Genome Biol. 2000;1(6):RESEARCH0011 PMID: 11178265
  35. Complete genome sequence of Caulobacter crescentus.
    Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4136-41 PMID: 11259647
  36. Establishing a direct role for the Bartonella bacilliformis invasion-associated locus B (IalB) protein in human erythrocyte parasitism.
    Infect Immun. 2001 Jul;69(7):4373-81 PMID: 11401976
  37. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae.
    Science. 2001 Jul 20;293(5529):498-506 PMID: 11463916
  38. The composite genome of the legume symbiont Sinorhizobium meliloti.
    Science. 2001 Jul 27;293(5530):668-72 PMID: 11474104
  39. The genome of the natural genetic engineer Agrobacterium tumefaciens C58.
    Science. 2001 Dec 14;294(5550):2317-23 PMID: 11743193
  40. The urease complex gene of Yersinia enterocolitica and its role in virulence.
    Contrib Microbiol Immunol. 1995;13:262-3 PMID: 8833849
  41. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
    Protein Eng. 1997 Jan;10(1):1-6 PMID: 9051728
  42. Microbial gene identification using interpolated Markov models.
    Nucleic Acids Res. 1998 Jan 15;26(2):544-8 PMID: 9421513
  43. Differences in chromosome number and genome rearrangements in the genus Brucella.
    Mol Microbiol. 1998 Jan;27(1):99-106 PMID: 9466259
  44. Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages.
    Infect Immun. 1998 Nov;66(11):5485-93 PMID: 9784561
  45. Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes.
    Infect Immun. 1998 Dec;66(12):5711-24 PMID: 9826346
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.192319099
Email
ipaulsen@tigr.org
Published
2002-10-01
Epub
2002-00-23
Pages
13148-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC130601
Subset
IM
Databases
PIR
AE014291, AE014292
Corrections
CommentIn
PMID: 12271145
External Links
PubMed source DOI: 10.1073/pnas.192319099 PMC Full Text View journal details
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