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PMID: 11756688 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The genome sequence of the facultative intracellular pathogen Brucella melitensis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 1 ·2002-01-08 ·Pages 443-8

DelVecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, Los T, Ivanova N, Anderson I, Bhattacharyya A, Lykidis A, Reznik G, Jablonski L, Larsen N, D'Souza M, Bernal A, Mazur M, Goltsman E, Selkov E, Elzer PH, Hagius S, O'Callaghan D, Letesson JJ, Haselkorn R, Kyrpides N, Overbeek R

Abstract

Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and Malta fever in humans. The genome of B. melitensis strain 16M was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 ORFs. By using the bioinformatics suite ERGO, 2,487 (78%) ORFs were assigned functions. The origins of replication of the two chromosomes are similar to those of other alpha-proteobacteria. Housekeeping genes, including those involved in DNA replication, transcription, translation, core metabolism, and cell wall biosynthesis, are distributed on both chromosomes. Type I, II, and III secretion systems are absent, but genes encoding sec-dependent, sec-independent, and flagella-specific type III, type IV, and type V secretion systems as well as adhesins, invasins, and hemolysins were identified. Several features of the B. melitensis genome are similar to those of the symbiotic Sinorhizobium meliloti.

MeSH Terms
Brucella melitensis/genetics Chromosomes Fatty Acids/metabolism Genome, Bacterial Models, Biological Models, Genetic Molecular Sequence Data Open Reading Frames Protein Biosynthesis Replication Origin Sequence Analysis, DNA Signal Transduction
Chemicals
Fatty Acids
Authors & Affiliations
25 authors, click to expand affiliations / ORCID
DelVecchio Vito G
Institute of Molecular Biology and Medicine, University of Scranton, Scranton, PA 18510, USA. vimbm@aol.com
Kapatral Vinayak
Redkar Rajendra J
Patra Guy
Mujer Cesar
Los Tamara
Ivanova Natalia
Anderson Iain
Bhattacharyya Anamitra
Lykidis Athanasios
Reznik Gary
Jablonski Lynn
Larsen Niels
D'Souza Mark
Bernal Axel
Mazur Mikhail
Goltsman Eugene
Selkov Eugene
Elzer Philip H
Hagius Sue
O'Callaghan David
Letesson Jean-Jacques
Haselkorn Robert
Kyrpides Nikos
Overbeek Ross
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.221575398
Email
vimbm@aol.com
Published
2002-01-08
Epub
2001-00-26
Pages
443-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC117579
Subset
IM
Databases
GENBANK
AE008917, AE008918
Corrections
CommentIn
PMID: 11782541
External Links
PubMed source DOI: 10.1073/pnas.221575398 PMC Full Text View journal details
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