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PMID: 11157954 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Potential symbiosis-specific genes uncovered by sequencing a 410-kilobase DNA region of the Bradyrhizobium japonicum chromosome.

Journal of bacteriology ·Vol. 183 ·No. 4 ·2001-02-00 ·Pages 1405-12

Göttfert M, Röthlisberger S, Kündig C, Beck C, Marty R, Hennecke H

Abstract

The physical and genetic map of the Bradyrhizobium japonicum chromosome revealed that nitrogen fixation and nodulation genes are clustered. Because of the complex interactions between the bacterium and the plant, we expected this chromosomal sector to contain additional genes that are involved in the maintenance of an efficient symbiosis. Therefore, we determined the nucleotide sequence of a 410-kb region. The overall G+C nucleotide content was 59.1%. Using a minimum gene length of 150 nucleotides, 388 open reading frames (ORFs) were selected as coding regions. Thirty-five percent of the predicted proteins showed similarity to proteins of rhizobia. Sixteen percent were similar only to proteins of other bacteria. No database match was found for 29%. Repetitive DNA sequence-derived ORFs accounted for the rest. The sequenced region contained all nitrogen fixation genes and, apart from nodM, all nodulation genes that were known to exist in B. japonicum. We found several genes that seem to encode transport systems for ferric citrate, molybdate, or carbon sources. Some of them are preceded by -24/-12 promoter elements. A number of putative outer membrane proteins and cell wall-modifying enzymes as well as a type III secretion system might be involved in the interaction with the host.

MeSH Terms
Acyltransferases/genetics Amidohydrolases/genetics Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/genetics,metabolism Bradyrhizobium/genetics Cations/metabolism Cell Wall/metabolism Chromosomes, Bacterial DNA, Bacterial Ferredoxins/genetics Genes, Bacterial Glucuronidase/genetics Metals/metabolism Models, Genetic Molecular Sequence Data N-Acetylglucosaminyltransferases/genetics Nitrogen Fixation/genetics Open Reading Frames Peptide Synthases/genetics Propanolamines/metabolism Recombination, Genetic/genetics Sequence Analysis, DNA/standards Symbiosis/genetics
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Cations DNA, Bacterial Ferredoxins Metals Propanolamines nodB protein, Bacteria Acyltransferases NodA protein, Rhizobiales N-Acetylglucosaminyltransferases NodC protein, Rhizobiales Glucuronidase Amidohydrolases Peptide Synthases rhizobitoxine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Göttfert M
Institut für Genetik, Technische Universität Dresden, D-01062 Dresden, Germany. mgoettfe@rcs.urz.tu-dresden.de
Röthlisberger S
Kündig C
Beck C
Marty R
Hennecke H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.4.1405-1412.2001
Email
mgoettfe@rcs.urz.tu-dresden.de
Published
2001-02-00
Pages
1405-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95015
Subset
IM
Databases
GENBANK
AF322012, AF322013
External Links
PubMed source DOI: 10.1128/JB.183.4.1405-1412.2001 PMC Full Text View journal details
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