Abstract
The gene for a new bacterial aquaporin, AqpX, was cloned from the pathogenic Gram-negative bacterium Brucella abortus. The gene was mapped on the large chromosome of B. abortus. It is flanked by one upstream and two downstream copies of the Brucella repeated sequence Bru-RS. Prediction from the nucleotide sequence indicated that the protein is a member of the MIP family, which comprises channels for water and/or solute transport. Expression in Xenopus oocytes and cryoelectron microscopy of Escherichia coli cells transformed with the aqpX gene confirmed that the protein is an efficient water channel. Glycerol uptake experiments in E. coli also showed that the protein is not able to transport glycerol.
MeSH Terms
Amino Acid Sequence
Animals
Aquaporins/chemistry,genetics,metabolism
Base Sequence
Brucella abortus/genetics,growth & development,metabolism
Brucellosis/microbiology,veterinary
Cattle
Chromosome Mapping/methods
Cloning, Molecular
Cryoelectron Microscopy
Escherichia coli/genetics,metabolism
Glycerol/metabolism
Molecular Sequence Data
Oocytes/physiology
Sequence Analysis, DNA
Water/metabolism
Xenopus/genetics,physiology
Chemicals
Aquaporins
Water
Glycerol
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rodrı Guez Marı A C
Departamento de Biologı́a Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad asociada al Centro de Investigaciones Biológicas, CSIC, Cardenal Herrera Oria s/n, 39011 Santander, Spain1.
Froger Alexandrine
UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
Rolland Jean-Paul
UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
Thomas Daniel
UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
Agüero Jesús
Departamento de Biologı́a Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad asociada al Centro de Investigaciones Biológicas, CSIC, Cardenal Herrera Oria s/n, 39011 Santander, Spain1.
Delamarche Christian
UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
Garcı A-Lobo Juan M
Departamento de Biologı́a Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad asociada al Centro de Investigaciones Biológicas, CSIC, Cardenal Herrera Oria s/n, 39011 Santander, Spain1.
