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PMID: 12186927 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The cauliflower mosaic virus virion-associated protein is dispensable for viral replication in single cells.

Journal of virology ·Vol. 76 ·No. 18 ·2002-09-00 ·Pages 9457-64

Kobayashi K, Tsuge S, Stavolone L, Hohn T

Abstract

Cauliflower mosaic virus (CaMV) open reading frame III (ORF III) codes for a virion-associated protein (Vap), which is one of two viral proteins essential for aphid transmission. However, unlike the aphid transmission factor encoded by CaMV ORF II, Vap is also essential for systemic infection, suggesting that it is a multifunctional protein. To elucidate the additional function or functions of Vap, we tested the replication of noninfectious ORF III-defective mutants in transfected turnip protoplasts. PCR and Western blot analyses revealed that CaMV replication had occurred with an efficiency similar to that of wild-type virus and without leading to reversions. Electron microscopic examination revealed that an ORF III frameshift mutant formed normally structured virions. These results demonstrate that Vap is dispensable for replication in single cells and is not essential for virion morphogenesis. Analysis of inoculated turnip leaves showed that the ORF III frameshift mutant does not cause any detectable local infection. These results are strongly indicative of a role for Vap in virus movement.

MeSH Terms
Amino Acid Sequence Brassica napus/virology Capsid/metabolism Caulimovirus/physiology DNA Replication Frameshift Mutation Gene Deletion Microscopy, Electron Molecular Sequence Data Open Reading Frames/genetics Plant Diseases/virology Plant Leaves/virology Polymerase Chain Reaction Protoplasts Transfection Viral Proteins/metabolism Virion/metabolism Virus Replication
Chemicals
Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kobayashi Kappei
Friedrich Miescher Institute, Basel, Switzerland.
Tsuge Seiji
Stavolone Livia
Hohn Thomas
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.18.9457-9464.2002
Published
2002-09-00
Pages
9457-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC136477
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.76.18.9457-9464.2002 PMC Full Text View journal details
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