A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA.

A simple and rapid procedure has been developed for the small-scale isolation of crude preparations of cauliflower mosaic virus DNA. The preparations are relatively free of contaminating cellular DNA and are sufficiently pure to be digested with restriction enzymes. The method enables plants infected with single-lesion isolates of mutagenized viral DNA to be rapidly screened for particular restriction endonuclease sites.