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PMID: 12003943 Published · ppublish English Comparative Study Journal Article

Characterization of the class 3 integron and the site-specific recombination system it determines.

Journal of bacteriology ·Vol. 184 ·No. 11 ·2002-06-00 ·Pages 3017-26

Collis CM, Kim MJ, Partridge SR, Stokes HW, Hall RM

Abstract

Integrons capture gene cassettes by using a site-specific recombination mechanism. As only one class of integron and integron-determined site-specific recombination system has been studied in detail, the properties of a second class, the only known class 3 integron, were examined. The configuration of the three potentially definitive features of integrons, the intI3 gene, the adjacent attI3 recombination site, and the P(c) promoter that directs transcription of the cassettes, was similar to that found in the corresponding region (5' conserved segment) of class 1 integrons. The integron features are flanked by a copy of the terminal inverted repeat, IRi, from class 1 integrons on one side and a resolvase-encoding tniR gene on the other, suggesting that they are part of a transposable element related to Tn402 but with the integron module in the opposite orientation. The IntI3 integrase was active and able to recognize and recombine both known types of IntI-specific recombination sites, the attI3 site in the integron, and different cassette-associated 59-be (59-base element) sites. Both integration of circularized cassettes into the attI3 site and excision of integrated cassettes were also catalyzed by IntI3. The attI3 site was localized to a short region adjacent to the intI3 gene. Recombination between a 59-be and secondary sites was also catalyzed by IntI3, but at frequencies significantly lower than observed with IntI1, the class 1 integron integrase.

MeSH Terms
Attachment Sites, Microbiological/genetics Base Sequence DNA Transposable Elements/genetics Drug Resistance Escherichia coli/enzymology,genetics Integrases/genetics,metabolism Molecular Sequence Data Promoter Regions, Genetic Recombination, Genetic Sequence Alignment Terminal Repeat Sequences
Chemicals
DNA Transposable Elements Integrases integron integrase IntI1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Collis Christina M
Sydney Laboratory, CSIRO Molecular Science, North Ryde, New South Wales 1670, Australia.
Kim Mi-Jurng
Partridge Sally R
Stokes H W
Hall Ruth M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.11.3017-3026.2002
Published
2002-06-00
Pages
3017-26
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135066
Subset
IM
Databases
GENBANK
AJ277063, D50438
External Links
PubMed source DOI: 10.1128/JB.184.11.3017-3026.2002 PMC Full Text View journal details
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