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PMID: 11973294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A role for histone H2B during repair of UV-induced DNA damage in Saccharomyces cerevisiae.

Genetics ·Vol. 160 ·No. 4 ·2002-04-00 ·Pages 1375-87

Martini EM, Keeney S, Osley MA

Abstract

To investigate the role of the nucleosome during repair of DNA damage in yeast, we screened for histone H2B mutants that were sensitive to UV irradiation. We have isolated a new mutant, htb1-3, that shows preferential sensitivity to UV-C. There is no detectable difference in bulk chromatin structure or in the number of UV-induced cis-syn cyclobutane pyrimidine dimers (CPD) between HTB1 and htb1-3 strains. These results suggest a specific effect of this histone H2B mutation in UV-induced DNA repair processes rather than a global effect on chromatin structure. We analyzed the UV sensitivity of double mutants that contained the htb1-3 mutation and mutations in genes from each of the three epistasis groups of RAD genes. The htb1-3 mutation enhanced UV-induced cell killing in rad1Delta and rad52Delta mutants but not in rad6Delta or rad18Delta mutants, which are defective in postreplicational DNA repair (PRR). When combined with other mutations that affect PRR, the histone mutation increased the UV sensitivity of strains with defects in either the error-prone (rev1Delta) or error-free (rad30Delta) branches of PRR, but did not enhance the UV sensitivity of a strain with a rad5Delta mutation. When combined with a ubc13Delta mutation, which is also epistatic with rad5Delta, the htb1-3 mutation enhanced UV-induced cell killing. These results suggest that histone H2B acts in a novel RAD5-dependent branch of PRR.

MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Chromatin/metabolism DNA Damage DNA Helicases DNA Repair/physiology DNA, Fungal/radiation effects Epistasis, Genetic Fungal Proteins/metabolism Histones/physiology Micrococcal Nuclease/metabolism Molecular Sequence Data Mutation Nucleosomes Pyrimidine Dimers Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Ultraviolet Rays
Chemicals
Chromatin DNA, Fungal Fungal Proteins Histones Nucleosomes Pyrimidine Dimers Saccharomyces cerevisiae Proteins Micrococcal Nuclease Adenosine Triphosphatases RAD5 protein, S cerevisiae DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martini Emmanuelle M D
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Keeney Scott
Osley Mary Ann
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/160.4.1375
Published
2002-04-00
Pages
1375-87
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462056
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040118 · United States
NIGMS NIH HHS · GM40118 · United States
NIGMS NIH HHS · GM58673 · United States
External Links
PubMed source DOI: 10.1093/genetics/160.4.1375 PMC Full Text View journal details
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