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PMID: 1193721 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Production of elevated levels of dextransucrase by a mutant of Streptococcus mutans.

Infection and immunity ·Vol. 12 ·No. 4 ·1975-10-00 ·Pages 934-7

Schachtele CF, Germaine GR, Harlander SK

Abstract

A mutant (S19) of Sreptococcus mutans strain 6715 which produces elevated levels of dextransucrase (EC 2.4.1.5) was isolated. Soluble enzyme in culture supernatant solutions from S19 polymerized the glucosyl moiety of sucrose into alcohol-insoluble and water-insoluble glucans at a rate three to six times greater than that of the parent strain. Washed-cell suspensions of S19 also contained increased amounts of cell-associated enzyme. Adherence of S19 to glass in the presence of sucrose occurred at twice the rate of strain 6715. The Km values for sucrose and primer dextran were similar for the mutant and parent enzymes. Mutant S19 should facilitate studies on the mechanism of adherence of S. Mutans and the control of dextransucrase production by this bacterium.

MeSH Terms
Glucosyltransferases/biosynthesis Mutation Streptococcus/enzymology Streptococcus mutans/enzymology,isolation & purification
Chemicals
Glucosyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schachtele C F
Germaine G R
Harlander S K
References (22)
22 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.12.4.934-937.1975
Published
1975-10-00
Pages
934-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415376
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.12.4.934-937.1975 PMC Full Text View journal details
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