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PMID: 4601768 Published · ppublish English Journal Article

Dissociation of plaque formation from glucan-induced agglutination in mutants of Streptococcus mutans.

Infection and immunity ·Vol. 10 ·No. 1 ·1974-07-00 ·Pages 189-96

Freedman ML, Tanzer JM

Abstract

The traits adhesion and cohesion are manifested in Streptococcus mutans by in vitro plaque formation and glucan-mediated agglutination and have been dissociated in mutants of this caries-associated microbe. In these mutants, which differ from each other in colonial morphology, we have observed a consistent loss of ability to form adhesive microbial deposits (plaque) on wires, but only a minimal alteration from the wild type in ability to agglutinate into macroscopic clumps in the presence of exogenous glucans or sucrose. Also, when these mutants were cultured in sucrose-containing medium, there was an increase in the synthesis of water-soluble, extracellular glucans and a decrease in the synthesis of alkali-soluble, cell-associated glucans. Based on these findings, it appears (i) that in vitro plaque formation and agglutination are distinct and dissociable traits, and (ii) that adhesion but not agglutination appears related to the synthesis of alkali-soluble, cell-associated glucans.

MeSH Terms
Agglutination Agglutination Tests Bacteriological Techniques Cell Adhesion Drug Resistance, Microbial Fermentation Glycogen/pharmacology Mutagens Mutation Nitrosoguanidines/pharmacology Polysaccharides, Bacterial/biosynthesis Streptococcus/isolation & purification,metabolism Streptomycin Sucrose/pharmacology Viral Plaque Assay
Chemicals
Mutagens Nitrosoguanidines Polysaccharides, Bacterial Sucrose Glycogen Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Freedman M L
Tanzer J M
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36 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.10.1.189-196.1974
Published
1974-07-00
Pages
189-96
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414976
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.10.1.189-196.1974 PMC Full Text View journal details
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