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PMID: 11864994 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin.

The Journal of cell biology ·Vol. 156 ·No. 5 ·2002-03-04 ·Pages 805-15

Lavoie BD, Hogan E, Koshland D

Abstract

The machinery mediating chromosome condensation is poorly understood. To begin to dissect the in vivo function(s) of individual components, we monitored mitotic chromosome structure in mutants of condensin, cohesin, histone H3, and topoisomerase II (topo II). In budding yeast, both condensation establishment and maintenance require all of the condensin subunits, but not topo II activity or phospho-histone H3. Structural maintenance of chromosome (SMC) protein 2, as well as each of the three non-SMC proteins (Ycg1p, Ycs4p, and Brn1p), was required for chromatin binding of the condensin complex in vivo. Using reversible condensin alleles, we show that chromosome condensation does not involve an irreversible modification of condensin or chromosomes. Finally, we provide the first evidence of a mechanistic link between condensin and cohesin function. A model discussing the functional interplay between cohesin and condensin is presented.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Carrier Proteins/genetics,metabolism Cell Cycle Proteins Cell Survival/genetics Chromosomal Proteins, Non-Histone Chromosome Segregation/physiology DNA Topoisomerases, Type II/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Fungal Proteins Histones/genetics,metabolism Mitosis/genetics,physiology Multiprotein Complexes Mutation/genetics Nuclear Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Carrier Proteins Cell Cycle Proteins Chromosomal Proteins, Non-Histone DNA-Binding Proteins Fungal Proteins Histones Multiprotein Complexes Nuclear Proteins SMC2 protein, S cerevisiae Saccharomyces cerevisiae Proteins cohesins condensin complexes Adenosine Triphosphatases DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lavoie Brigitte D
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON M5S 1A8, Canada. brigitte.lavoie@utoronto.ca
Hogan Eileen
Koshland Douglas
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200109056
Email
brigitte.lavoie@utoronto.ca
Published
2002-03-04
Epub
2002-00-25
Pages
805-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173308
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200109056 PMC Full Text View journal details
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