Home LiteratureArticle Details
PMID: 9335334 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.

Cell ·Vol. 91 ·No. 1 ·1997-10-03 ·Pages 47-57

Guacci V, Koshland D, Strunnikov A

Abstract

The S. cerevisiae MCD1 (mitotic chromosome determinant) gene was identified in genetic screens for genes important for chromosome structure. MCD1 is essential for viability and homologs are found from yeast to humans. Analysis of the mcd1 mutant and cell cycle-dependent expression pattern of Mcd1p suggest that this protein functions in chromosome morphogenesis from S phase through mitosis. The mcd1 mutant is defective in sister chromatid cohesion and chromosome condensation. The physical association between Mcd1p and Smc1p, one of the SMC family of chromosomal proteins, further suggests that Mcd1p functions directly on chromosomes. These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link.

MeSH Terms
Cell Cycle Proteins/analysis,genetics,physiology Cell Division Cell Nucleus/chemistry Chromatids/physiology Chromosomal Proteins, Non-Histone Chromosomes, Fungal/physiology Fungal Proteins/genetics Gene Expression Regulation, Fungal/physiology Genes, Fungal/genetics Models, Genetic Mutation Nuclear Proteins Phosphoproteins RNA, Fungal/analysis RNA, Messenger/analysis Recombinant Fusion Proteins Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Cell Cycle Proteins Chromosomal Proteins, Non-Histone Fungal Proteins MCD1 protein, S cerevisiae Nuclear Proteins Phosphoproteins RNA, Fungal RNA, Messenger Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins structural maintenance of chromosome protein 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guacci V
Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA.
Koshland D
Strunnikov A
References (51)
51 references, click to expand
  1. Cut2 proteolysis required for sister-chromatid seperation in fission yeast.
    Nature. 1996 May 30;381(6581):438-41 PMID: 8632802
  2. Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression.
    Cell. 1996 Jul 26;86(2):275-85 PMID: 8706132
  3. SMC proteins constitute two subunits of the mammalian recombination complex RC-1.
    EMBO J. 1996 Aug 1;15(15):4061-8 PMID: 8670910
  4. PEST sequences and regulation by proteolysis.
    Trends Biochem Sci. 1996 Jul;21(7):267-71 PMID: 8755249
  5. Structure and functions of the 20S and 26S proteasomes.
    Annu Rev Biochem. 1996;65:801-47 PMID: 8811196
  6. Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae.
    Genes Dev. 1996 Oct 15;10(20):2564-76 PMID: 8895658
  7. Mitotic chromosome condensation.
    Annu Rev Cell Dev Biol. 1996;12:305-33 PMID: 8970729
  8. Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
    Genes Dev. 1996 Dec 15;10(24):3081-93 PMID: 8985178
  9. GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion.
    Curr Biol. 1996 Dec 1;6(12):1599-608 PMID: 8994824
  10. Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast.
    Curr Biol. 1996 Dec 1;6(12):1609-20 PMID: 8994825
  11. Molecular model for telomeric heterochromatin in yeast.
    Curr Opin Cell Biol. 1997 Jun;9(3):383-7 PMID: 9159071
  12. Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
    Cell. 1997 May 16;89(4):511-21 PMID: 9160743
  13. Centromere position in budding yeast: evidence for anaphase A.
    Mol Biol Cell. 1997 Jun;8(6):957-72 PMID: 9201708
  14. Mis6, a fission yeast inner centromere protein, acts during G1/S and forms specialized chromatin required for equal segregation.
    Cell. 1997 Jul 11;90(1):131-43 PMID: 9230309
  15. Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
    Cell. 1997 Oct 3;91(1):35-45 PMID: 9335333
  16. Mouse satellite DNA, centromere structure, and sister chromatid pairing.
    J Cell Biol. 1986 Oct;103(4):1145-51 PMID: 2429969
  17. Chromosome condensation and decondensation factors in the life cycle of eukaryotic cells.
    Symp Fundam Cancer Res. 1984;37:45-69 PMID: 6528128
  18. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
    J Cell Biol. 1984 Mar;98(3):922-33 PMID: 6365930
  19. The structure of histone-depleted metaphase chromosomes.
    Cell. 1977 Nov;12(3):817-28 PMID: 922894
  20. Role of nonhistone proteins in metaphase chromosome structure.
    Cell. 1977 Nov;12(3):805-16 PMID: 922893
  21. Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).
    J Cell Biol. 1996 Apr;133(1):99-110 PMID: 8601617
  22. Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae.
    J Cell Biol. 1996 Apr;133(1):85-97 PMID: 8601616
  23. cDNA cloning and sequence analysis of a novel calcium binding protein with oligoproline motif.
    Cell Struct Funct. 1995 Aug;20(4):263-8 PMID: 8521526
  24. The rad21 gene product of Schizosaccharomyces pombe is a nuclear, cell cycle-regulated phosphoprotein.
    J Biol Chem. 1995 Mar 31;270(13):7703-11 PMID: 7706319
  25. Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions.
    Cell. 1995 Oct 20;83(2):247-56 PMID: 7585942
  26. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1.
    DNA Res. 1994;1(5):223-9 PMID: 7584044
  27. SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
    Genes Dev. 1995 Mar 1;9(5):587-99 PMID: 7698648
  28. Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
    EMBO J. 1994 Oct 17;13(20):4938-52 PMID: 7957061
  29. Structure and function of chromosomes in mitosis of budding yeast.
    Cold Spring Harb Symp Quant Biol. 1993;58:677-85 PMID: 7956084
  30. DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome.
    Cell. 1994 Nov 4;79(3):459-74 PMID: 7954812
  31. Chromosome segregation in mitosis and meiosis.
    Annu Rev Cell Biol. 1985;1:289-315 PMID: 3916318
  32. The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.
    J Cell Biol. 1987 Nov;105(5):2053-67 PMID: 3316246
  33. Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line.
    Cell. 1988 Jan 15;52(1):51-61 PMID: 2830981
  34. Detection of distinct structural domains within the primary constriction using autoantibodies.
    Chromosoma. 1988;96(5):360-7 PMID: 3044705
  35. The metaphase scaffold is helically folded: sister chromatids have predominantly opposite helical handedness.
    Cell. 1988 Dec 23;55(6):937-44 PMID: 2849511
  36. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  37. The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
    Cell. 1989 Jun 16;57(6):997-1007 PMID: 2544298
  38. Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Jan;11(1):329-37 PMID: 1986229
  39. Genes occupy a fixed and symmetrical position on sister chromatids.
    Cell. 1991 Feb 22;64(4):761-6 PMID: 1997205
  40. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function.
    Cell. 1991 Aug 9;66(3):507-17 PMID: 1651171
  41. Feedback control of mitosis in budding yeast.
    Cell. 1991 Aug 9;66(3):519-31 PMID: 1651172
  42. Scanning electron microscopy of mammalian chromosomes from prophase to telophase.
    Chromosoma. 1991 Jul;100(6):410-8 PMID: 1893796
  43. Delineation of DNA replication time zones by fluorescence in situ hybridization.
    EMBO J. 1992 Mar;11(3):1217-25 PMID: 1547781
  44. A simple and highly efficient procedure for rescuing autonomous plasmids from yeast.
    Nucleic Acids Res. 1992 Jul 25;20(14):3790 PMID: 1641351
  45. Cloning and characterization of rad21 an essential gene of Schizosaccharomyces pombe involved in DNA double-strand-break repair.
    Nucleic Acids Res. 1992 Dec 25;20(24):6605-11 PMID: 1480481
  46. Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
    Cell. 1993 Jul 2;73(7):1393-402 PMID: 8391932
  47. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1993 Jul 11;21(14):3329-30 PMID: 8341614
  48. SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.
    J Cell Biol. 1993 Dec;123(6 Pt 2):1635-48 PMID: 8276886
  49. Chromosome condensation and sister chromatid pairing in budding yeast.
    J Cell Biol. 1994 May;125(3):517-30 PMID: 8175878
  50. ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.
    J Cell Biol. 1994 Oct;127(2):303-18 PMID: 7929577
  51. A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
    Cell. 1994 Nov 4;79(3):449-58 PMID: 7954811
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/s0092-8674(01)80008-8
Published
1997-10-03
Pages
47-57
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2670185
Subset
IM
Grants
Intramural NIH HHS · Z01 HD001903-11 · United States
Intramural NIH HHS · Z99 AI999999 · United States
NIGMS NIH HHS · GM41718 · United States
Corrections
CommentIn
PMID: 9335328
External Links
PubMed source DOI: 10.1016/s0092-8674(01)80008-8 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2