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PMID: 11606525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae.

Genetics ·Vol. 159 ·No. 2 ·2001-10-00 ·Pages 453-70

Biggins S, Bhalla N, Chang A, Smith DL, Murray AW

Abstract

Accurate chromosome segregation requires the precise coordination of events during the cell cycle. Replicated sister chromatids are held together while they are properly attached to and aligned by the mitotic spindle at metaphase. At anaphase, the links between sisters must be promptly dissolved to allow the mitotic spindle to rapidly separate them to opposite poles. To isolate genes involved in chromosome behavior during mitosis, we microscopically screened a temperature-sensitive collection of budding yeast mutants that contain a GFP-marked chromosome. Nine LOC (loss of cohesion) complementation groups that do not segregate sister chromatids at anaphase were identified. We cloned the corresponding genes and performed secondary tests to determine their function in chromosome behavior. We determined that three LOC genes, PDS1, ESP1, and YCS4, are required for sister chromatid separation and three other LOC genes, CSE4, IPL1, and SMT3, are required for chromosome segregation. We isolated alleles of two genes involved in splicing, PRP16 and PRP19, which impair alpha-tubulin synthesis thus preventing spindle assembly, as well as an allele of CDC7 that is defective in DNA replication. We also report an initial characterization of phenotypes associated with the SMT3/SUMO gene and the isolation of WSS1, a high-copy smt3 suppressor.

MeSH Terms
Alleles Base Sequence Chromatids Chromosomes, Fungal Cloning, Molecular DNA Primers Genes, Fungal Genes, Suppressor Genetic Complementation Test Mutation Saccharomyces cerevisiae/genetics
Chemicals
DNA Primers
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Biggins S
Department of Physiology, University of California, San Francisco, 94143, USA. sbiggins@fhcrc.org
Bhalla N
Chang A
Smith D L
Murray A W
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/159.2.453
Email
sbiggins@fhcrc.org
Published
2001-10-00
Pages
453-70
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461834
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/159.2.453 PMC Full Text View journal details
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