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PMID: 11705902 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Fish monocytes as a model for mycobacterial host-pathogen interactions.

Infection and immunity ·Vol. 69 ·No. 12 ·2001-12-00 ·Pages 7310-7

El-Etr SH, Yan L, Cirillo JD

Abstract

Mycobacterium marinum, a relatively rapid-growing fish and human pathogen, has become an important model for the investigation of mycobacterial pathogenesis. M. marinum is closely related to the Mycobacterium tuberculosis complex and causes a disease in fish and amphibians with pathology similar to tuberculosis. We have developed an in vitro model for the study of M. marinum virulence mechanisms using the carp monocytic cell line CLC (carp leukocyte culture). We found that fish monocytes can differentiate between pathogenic and nonpathogenic mycobacterial species. Interestingly, M. marinum enters fish monocytes at a 40- to 60-fold-higher rate than Mycobacterium smegmatis. In addition, M. marinum survives and replicates in fish monocytes while M. smegmatis is killed. We also found that M. marinum inhibits lysosomal fusion in fish monocytes, indicating that these cells may be used to dissect the mechanisms of intracellular trafficking in mycobacteria. We conclude from these observations that monocytic cells from fish, a natural host for M. marinum, provide an extremely valuable model for the identification and characterization of mycobacterial virulence determinants in the laboratory.

MeSH Terms
Animals Bacterial Adhesion Carps/microbiology Cell Line Lysosomes/microbiology Macrophages/microbiology Membrane Fusion Mice Monocytes/cytology,microbiology Mycobacterium marinum/pathogenicity Mycobacterium smegmatis/pathogenicity Species Specificity Vacuoles/microbiology
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
El-Etr S H
Department of Veterinary and Biomedical Sciences, University of Nebraska at Lincoln, 68583-0905, USA.
Yan L
Cirillo J D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.69.12.7310-7317.2001
Published
2001-12-00
Pages
7310-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC98816
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.69.12.7310-7317.2001 PMC Full Text View journal details
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