Home LiteratureArticle Details
PMID: 11595802 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.

The Plant cell ·Vol. 13 ·No. 10 ·2001-10-00 ·Pages 2283-95

Baldwin TC, Handford MG, Yuseff MI, Orellana A, Dupree P

Abstract

Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.

MeSH Terms
Amino Acid Sequence Arabidopsis/cytology,genetics,growth & development Arabidopsis Proteins/chemistry,genetics,metabolism Base Sequence Carrier Proteins/chemistry,genetics,metabolism DNA Primers Glycosylation Golgi Apparatus/metabolism Intracellular Membranes/metabolism Membrane Transport Proteins Microscopy, Confocal Molecular Sequence Data Protein Conformation Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Arabidopsis Proteins Carrier Proteins DNA Primers GDP-mannose transporter GONST1 protein, Arabidopsis Membrane Transport Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baldwin T C
Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, United Kingdom.
Handford M G
Yuseff M I
Orellana A
Dupree P
References (60)
60 references, click to expand
  1. SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N- acetylgalactosamine, and UDP-galactose.
    Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3738-43 PMID: 11259660
  2. A proteomic analysis of organelles from Arabidopsis thaliana.
    Electrophoresis. 2000 Oct;21(16):3488-99 PMID: 11079568
  3. Splice site prediction in Arabidopsis thaliana pre-mRNA by combining local and global sequence information.
    Nucleic Acids Res. 1996 Sep 1;24(17):3439-52 PMID: 8811101
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. Getting started with yeast.
    Methods Enzymol. 1991;194:3-21 PMID: 2005794
  6. Brefeldin A Effects in Plants (Are Different Golgi Responses Caused by Different Sites of Action?).
    Plant Physiol. 1997 Jun;114(2):401-403 PMID: 12223714
  7. Identification of a conserved motif in the yeast golgi GDP-mannose transporter required for binding to nucleotide sugar.
    J Biol Chem. 2001 Feb 9;276(6):4424-32 PMID: 11067855
  8. Subcellular localization of sugar nucleotide synthetases.
    J Biol Chem. 1980 Oct 10;255(19):9225-9 PMID: 6251080
  9. A specialized pathway affecting virulence glycoconjugates of Leishmania.
    Science. 1995 Sep 29;269(5232):1869-72 PMID: 7569927
  10. The plant Golgi apparatus.
    Biochim Biophys Acta. 1998 Aug 14;1404(1-2):259-70 PMID: 9714825
  11. Evidence for a UDP-Glucose Transporter in Golgi Apparatus-Derived Vesicles from Pea and Its Possible Role in Polysaccharide Biosynthesis.
    Plant Physiol. 1996 Dec;112(4):1585-1594 PMID: 12226465
  12. Effect of brefeldin A on the structure of the Golgi apparatus and on the synthesis and secretion of proteins and polysaccharides in sycamore maple (Acer pseudoplatanus) suspension-cultured cells.
    Plant Physiol. 1993 Apr;101(4):1363-73 PMID: 8310065
  13. The yeast VRG4 gene is required for normal Golgi functions and defines a new family of related genes.
    J Biol Chem. 1996 Feb 16;271(7):3837-45 PMID: 8632002
  14. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  15. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
    Plant J. 1998 Dec;16(6):735-43 PMID: 10069079
  16. The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast.
    J Biol Chem. 1993 Mar 5;268(7):4953-62 PMID: 8444873
  17. The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose-4,6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose.
    Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):2085-90 PMID: 9050909
  18. Transporters of nucleotide sugars, nucleotide sulfate and ATP in the Golgi apparatus.
    Trends Biochem Sci. 1997 Jun;22(6):203-7 PMID: 9204706
  19. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  20. Transporters of nucleotide sugars, ATP, and nucleotide sulfate in the endoplasmic reticulum and Golgi apparatus.
    Annu Rev Biochem. 1998;67:49-69 PMID: 9759482
  21. The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae.
    J Biol Chem. 1997 Dec 12;272(50):31908-14 PMID: 9395539
  22. Yeast glycosylation mutants are sensitive to aminoglycosides.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1287-91 PMID: 7877969
  23. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  24. Distinct protein domains of the yeast Golgi GDP-mannose transporter mediate oligomer assembly and export from the endoplasmic reticulum.
    J Biol Chem. 2000 Jun 9;275(23):17718-27 PMID: 10748175
  25. Sequence and domain structure of talin.
    Nature. 1990 Oct 18;347(6294):685-9 PMID: 2120593
  26. Transport of UDP-galactose into the Golgi lumen regulates the biosynthesis of proteoglycans.
    J Biol Chem. 1996 Feb 16;271(7):3897-901 PMID: 8632010
  27. Mechanisms of glycosylation and sulfation in the Golgi apparatus: evidence for nucleotide sugar/nucleoside monophosphate and nucleotide sulfate/nucleoside monophosphate antiports in the Golgi apparatus membrane.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7051-5 PMID: 6095266
  28. How the cell wall acquired a cellular context.
    Plant Physiol. 2001 Jan;125(1):127-30 PMID: 11154314
  29. Vanadate-resistant yeast mutants are defective in protein glycosylation.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3209-12 PMID: 2014241
  30. floricaula: a homeotic gene required for flower development in antirrhinum majus.
    Cell. 1990 Dec 21;63(6):1311-22 PMID: 1702033
  31. Brefeldin A effects in plant and fungal cells: something new about vesicle trafficking?
    J Microsc. 1996 Feb;181(Pt 2):162-77 PMID: 8919983
  32. The Leishmania GDP-mannose transporter is an autonomous, multi-specific, hexameric complex of LPG2 subunits.
    Biochemistry. 2000 Feb 29;39(8):2013-22 PMID: 10684651
  33. Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases.
    Glycobiology. 1996 Jan;6(1):73-81 PMID: 8991513
  34. Expression cloning of the Golgi CMP-sialic acid transporter.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7572-6 PMID: 8755516
  35. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  36. Metabolism of uridine 5'-diphosphate-glucose in golgi vesicles from pea stems
    Plant Physiol. 1998 Jul;117(3):1007-14 PMID: 9662543
  37. Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity.
    EMBO J. 1998 Jan 15;17(2):423-34 PMID: 9430634
  38. Topography of glycosylation in yeast: characterization of GDPmannose transport and lumenal guanosine diphosphatase activities in Golgi-like vesicles.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6935-9 PMID: 2476806
  39. The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals.
    Proc Natl Acad Sci U S A. 1997 May 27;94(11):5679-84 PMID: 9159132
  40. Molecular cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter from Kluyveromyces lactis.
    Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5963-8 PMID: 8650202
  41. Mutants of Arabidopsis thaliana with altered cell wall polysaccharide composition.
    Plant J. 1997 Aug;12(2):335-45 PMID: 9301086
  42. Prediction of complete gene structures in human genomic DNA.
    J Mol Biol. 1997 Apr 25;268(1):78-94 PMID: 9149143
  43. G.A.T.T. (A General Agreement on Traffic and Transport) and Brefeldin A in Plant Cells.
    Plant Cell. 1994 Apr;6(4):463-467 PMID: 12244244
  44. Sugar residues on proteins.
    CRC Crit Rev Biochem. 1981;10(4):307-77 PMID: 6265145
  45. The N-terminal 77 amino acids from tobacco N-acetylglucosaminyltransferase I are sufficient to retain a reporter protein in the Golgi apparatus of Nicotiana benthamiana cells.
    FEBS Lett. 1999 Jun 18;453(1-2):169-73 PMID: 10403396
  46. Nucleotide sugar transporters of the Golgi apparatus.
    Curr Opin Struct Biol. 2000 Oct;10(5):542-7 PMID: 11042451
  47. Plant glycosyltransferases.
    Curr Opin Plant Biol. 2001 Jun;4(3):219-24 PMID: 11312132
  48. Golgi GDP-mannose uptake requires Leishmania LPG2. A member of a eukaryotic family of putative nucleotide-sugar transporters.
    J Biol Chem. 1997 Feb 7;272(6):3799-805 PMID: 9013638
  49. The ins(ide) and out(side) of dolichyl phosphate biosynthesis and recycling in the endoplasmic reticulum.
    Glycobiology. 2001 May;11(5):61R-70R PMID: 11425794
  50. The gene defective in leukocyte adhesion deficiency II encodes a putative GDP-fucose transporter.
    Nat Genet. 2001 May;28(1):69-72 PMID: 11326279
  51. Targeting of active sialyltransferase to the plant Golgi apparatus.
    Plant Cell. 1998 Oct;10(10):1759-68 PMID: 9761801
  52. A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes.
    Plant J. 1998 Nov;16(3):393-401 PMID: 9881160
  53. GDP-fucose uptake into the Golgi apparatus during xyloglucan biosynthesis requires the activity of a transporter-like protein other than the UDP-glucose transporter.
    Plant Physiol. 2000 Mar;122(3):867-77 PMID: 10712551
  54. Glucomannan synthesis in pea epicotyls: the mannose and glucose transferases.
    Planta. 1993;190(2):206-20 PMID: 7685647
  55. Stop-and-go movements of plant Golgi stacks are mediated by the acto-myosin system.
    Plant Physiol. 1999 Dec;121(4):1127-42 PMID: 10594100
  56. Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network.
    Plant J. 1998 Aug;15(3):441-7 PMID: 9750355
  57. Identification and purification of the rat liver Golgi membrane UDP-N-acetylgalactosamine transporter.
    J Biol Chem. 1999 Feb 12;274(7):4474-9 PMID: 9933652
  58. Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein.
    J Biol Chem. 1988 Nov 25;263(33):17499-507 PMID: 3053713
  59. Matrix polysaccharide precursors in Arabidopsis cell walls are synthesized by alternate pathways with organ-specific expression patterns.
    Plant J. 2000 Mar;21(6):537-46 PMID: 10758504
  60. Locating protein-coding regions in human DNA sequences by a multiple sensor-neural network approach.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11261-5 PMID: 1763041
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010247
Published
2001-10-00
Pages
2283-95
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139159
Subset
IM
Databases
GENBANK
AJ314836
External Links
PubMed source DOI: 10.1105/tpc.010247 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2