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PMID: 11259660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N- acetylgalactosamine, and UDP-galactose.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 7 ·2001-03-27 ·Pages 3738-43

Berninsone P, Hwang HY, Zemtseva I, Horvitz HR, Hirschberg CB

Abstract

Caenorhabditis elegans sqv mutants are defective in vulval epithelial invagination and have a severe reduction in hermaphrodite fertility. The gene sqv-7 encodes a multitransmembrane hydrophobic protein resembling nucleotide sugar transporters of the Golgi membrane. A Golgi vesicle enriched fraction of Saccharomyces cerevisiae expressing SQV-7 transported UDP-glucuronic acid, UDP-N-acetylgalactosamine, and UDP-galactose (Gal) in a temperature-dependent and saturable manner. These nucleotide sugars are competitive, alternate, noncooperative substrates. The two mutant sqv-7 missense alleles resulted in a severe reduction of these three transport activities. SQV-7 did not transport CMP-sialic acid, GDP-fucose, UDP-N-acetylglucosamine, UDP-glucose, or GDP-mannose. SQV-7 is able to transport UDP-Gal in vivo, as shown by its ability to complement the phenotype of Madin-Darby canine kidney ricin resistant cells, a mammalian cell line deficient in UDP-Gal transport into the Golgi. These results demonstrate that unlike most nucleotide sugar transporters, SQV-7 can transport multiple distinct nucleotide sugars. We propose that SQV-7 translocates multiple nucleotide sugars into the Golgi lumen for the biosynthesis of glycoconjugates that play a pivotal role in development.

MeSH Terms
Animals Biological Transport Caenorhabditis elegans/embryology,metabolism Caenorhabditis elegans Proteins Carrier Proteins/metabolism,physiology Cells, Cultured Dogs Drug Resistance Epithelial Cells/physiology Monosaccharide Transport Proteins Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins Saccharomyces cerevisiae Subcellular Fractions Transfection Uridine Diphosphate Galactose/metabolism Uridine Diphosphate Glucuronic Acid/metabolism Uridine Diphosphate N-Acetylgalactosamine/metabolism
Chemicals
Caenorhabditis elegans Proteins Carrier Proteins Monosaccharide Transport Proteins Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins sqv-7 protein, C elegans Uridine Diphosphate Glucuronic Acid Uridine Diphosphate Galactose Uridine Diphosphate N-Acetylgalactosamine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Berninsone P
Department of Molecular and Cell Biology, Boston University School of Dental Medicine, Boston, MA 02118, USA.
Hwang H Y
Zemtseva I
Horvitz H R
Hirschberg C B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.061593098
Published
2001-03-27
Epub
2001-00-20
Pages
3738-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC31122
Subset
IM
Grants
NIGMS NIH HHS · GM 30365 · United States
NIGMS NIH HHS · R01 GM024663 · United States
NIGMS NIH HHS · R01 GM030365 · United States
NIGMS NIH HHS · R37 GM024663 · United States
NIGMS NIH HHS · GM 24663 · United States
NIGMS NIH HHS · R37 GM030365 · United States
NIGMS NIH HHS · GM 34396 · United States
External Links
PubMed source DOI: 10.1073/pnas.061593098 PMC Full Text View journal details
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