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PMID: 11591679 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interactive control of Rhodobacter capsulatus redox-balancing systems during phototrophic metabolism.

Journal of bacteriology ·Vol. 183 ·No. 21 ·2001-11-00 ·Pages 6344-54

Tichi MA, Tabita FR

Abstract

In nonsulfur purple bacteria, redox homeostasis is achieved by the coordinate control of various oxidation-reduction balancing mechanisms during phototrophic anaerobic respiration. In this study, the ability of Rhodobacter capsulatus to maintain a balanced intracellular oxidation-reduction potential was considered; in addition, interrelationships between the control of known redox-balancing systems, the Calvin-Benson-Bassham, dinitrogenase and dimethyl sulfoxide reductase systems, were probed in strains grown under both photoheterotrophic and photoautotrophic growth conditions. By using cbb(I) (cbb form I operon)-, cbb(II)-, nifH-, and dorC-reporter gene fusions, it was demonstrated that each redox-balancing system responds to specific metabolic circumstances under phototrophic growth conditions. In specific mutant strains of R. capsulatus, expression of both the Calvin-Benson-Bassham and dinitrogenase systems was influenced by dimethyl sulfoxide respiration. Under photoheterotrophic growth conditions, coordinate control of redox-balancing systems was further manifested in ribulose 1,5-bisphosphate carboxylase/oxygenase and phosphoribulokinase deletion strains. These findings demonstrated the existence of interactive control mechanisms that govern the diverse means by which R. capsulatus maintains redox poise during photoheterotrophic and photoautotrophic growth.

MeSH Terms
Bacterial Proteins/metabolism Carbon/metabolism Gene Expression Regulation, Bacterial Genes, Reporter Homeostasis Iron-Sulfur Proteins Models, Biological Mutation Nitrogenase/metabolism Oxidation-Reduction Oxidoreductases/metabolism Pentose Phosphate Pathway Photosynthesis Promoter Regions, Genetic Rhodobacter capsulatus/genetics,growth & development,metabolism
Chemicals
Bacterial Proteins Iron-Sulfur Proteins Carbon Oxidoreductases Nitrogenase dimethyl sulfoxide reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tichi M A
Department of Microbiology and Plant Molecular Biology/Biotechnology Program, The Ohio State University, Columbus, Ohio 43210-1292, USA.
Tabita F R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.21.6344-6354.2001
Published
2001-11-00
Pages
6344-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC100130
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045404 · United States
NIGMS NIH HHS · GM45404 · United States
External Links
PubMed source DOI: 10.1128/JB.183.21.6344-6354.2001 PMC Full Text View journal details
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