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PMID: 11543653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Reduced macrophage infiltration and demyelination in mice lacking the chemokine receptor CCR5 following infection with a neurotropic coronavirus.

Virology ·Vol. 288 ·No. 1 ·2001-09-15 ·Pages 8-17

Glass WG, Liu MT, Kuziel WA, Lane TE

Abstract

Studies were performed to investigate the contributions of the CC chemokine receptor CCR5 in host defense and disease development following intracranial infection with mouse hepatitis virus (MHV). T cell recruitment was impaired in MHV-infected CCR5(-/-) mice at day 7 postinfection (pi), which correlated with increased (P < or = 0.03) titers within the brain. However, by day 12 pi, T cell infiltration into the CNS of infected CCR5(-/-) and CCR5(+/+) mice was similar and both strains exhibited comparable viral titers, indicating that CCR5 expression is not essential for host defense. Following MHV infection of CCR5(+/+) mice, greater than 50% of cells expressing CCR5 antigen were activated macrophage/microglia (determined by F4/80 antigen expression). In addition, infected CCR5(-/-) mice exhibited reduced (P < or = 0.02) macrophage (CD45(high)F4/80(+)) infiltration, which correlated with a significant reduction (P < or = 0.001) in the severity of demyelination compared to CCR5(+/+) mice. These data indicate that CCR5 contributes to MHV-induced demyelination by allowing macrophages to traffic into the CNS.

MeSH Terms
Animals Brain/pathology,virology Coronavirus Infections/immunology,pathology Encephalitis, Viral/immunology,pathology Hepatitis, Viral, Animal/immunology,pathology Macrophages/physiology Mice Mice, Inbred C57BL Mice, Knockout Murine hepatitis virus/isolation & purification,physiology Myelin Sheath/pathology Receptors, CCR5/deficiency,genetics,physiology T-Lymphocytes/immunology Virus Replication
Chemicals
Receptors, CCR5
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Glass W G
Department of Molecular Biology and Biochemistry, University of California, Irvine, 92697-3900, USA.
Liu M T
Kuziel W A
Lane T E
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1006/viro.2001.1050
Published
2001-09-15
Pages
8-17
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7142305
Subset
IM
Grants
PHS HHS · 37336-01 · United States
NIAID NIH HHS · AI07319-12 · United States
NINDS NIH HHS · T32NS07444 · United States
External Links
PubMed source DOI: 10.1006/viro.2001.1050 PMC Full Text View journal details
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