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PMID: 11514613 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Caveolin-1 expression negatively regulates cell cycle progression by inducing G(0)/G(1) arrest via a p53/p21(WAF1/Cip1)-dependent mechanism.

Molecular biology of the cell ·Vol. 12 ·No. 8 ·2001-08-00 ·Pages 2229-44

Galbiati F, Volonté D, Liu J, Capozza F, Frank PG, Zhu L, Pestell RG, Lisanti MP

Abstract

Caveolin-1 is a principal component of caveolae membranes in vivo. Caveolin-1 mRNA and protein expression are lost or reduced during cell transformation by activated oncogenes. Interestingly, the human caveolin-1 gene is localized to a suspected tumor suppressor locus (7q31.1). However, it remains unknown whether caveolin-1 plays any role in regulating cell cycle progression. Here, we directly demonstrate that caveolin-1 expression arrests cells in the G(0)/G(1) phase of the cell cycle. We show that serum starvation induces up-regulation of endogenous caveolin-1 and arrests cells in the G(0)/G(1) phase of the cell cycle. Moreover, targeted down-regulation of caveolin-1 induces cells to exit the G(0)/G(1) phase. Next, we constructed a green fluorescent protein-tagged caveolin-1 (Cav-1-GFP) to examine the effect of caveolin-1 expression on cell cycle regulation. We directly demonstrate that recombinant expression of Cav-1-GFP induces arrest in the G(0)/G(1) phase of the cell cycle. To examine whether caveolin-1 expression is important for modulating cell cycle progression in vivo, we expressed wild-type caveolin-1 as a transgene in mice. Analysis of primary cultures of mouse embryonic fibroblasts from caveolin-1 transgenic mice reveals that caveolin-1 induces 1) cells to exit the S phase of the cell cycle with a concomitant increase in the G(0)/G(1) population, 2) a reduction in cellular proliferation, and 3) a reduction in the DNA replication rate. Finally, we demonstrate that caveolin-1-mediated cell cycle arrest occurs through a p53/p21-dependent pathway. Taken together, our results provide the first evidence that caveolin-1 expression plays a critical role in the modulation of cell cycle progression in vivo.

MeSH Terms
Animals Caspase 3 Caspases/metabolism Caveolin 1 Caveolins/metabolism Cell Cycle/physiology Cell Separation Cells, Cultured Culture Media, Serum-Free Cyclin-Dependent Kinase Inhibitor p21 Cyclins/metabolism Enzyme Inhibitors/metabolism Female Fibroblasts/drug effects,metabolism Flow Cytometry Genes, Reporter Humans Immunoblotting Male Mice Mice, Inbred C57BL Mice, Transgenic Pregnancy Recombinant Fusion Proteins/genetics,metabolism Staurosporine/pharmacology Tumor Suppressor Protein p53/metabolism
Chemicals
CAV1 protein, human CDKN1A protein, human Cav1 protein, mouse Caveolin 1 Caveolins Cdkn1a protein, mouse Culture Media, Serum-Free Cyclin-Dependent Kinase Inhibitor p21 Cyclins Enzyme Inhibitors Recombinant Fusion Proteins Tumor Suppressor Protein p53 CASP3 protein, human Casp3 protein, mouse Caspase 3 Caspases Staurosporine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Galbiati F
Department of Molecular Pharmacology and The Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Volonté D
Liu J
Capozza F
Frank P G
Zhu L
Pestell R G
Lisanti M P
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
Published
2001-08-00
Pages
2229-44
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC58591
Subset
IM
Grants
NHLBI NIH HHS · P50-HL-56399 · United States
NCI NIH HHS · R01 CA075503 · United States
NCI NIH HHS · R01-CA-70897 · United States
Telethon · 470/BI · Italy
NCI NIH HHS · R01-CA-75503 · United States
NHLBI NIH HHS · P50 HL056399 · United States
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