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PMID: 11486018 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of the Srb10 kinase with Sip4, a transcriptional activator of gluconeogenic genes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 21 ·No. 17 ·2001-09-00 ·Pages 5790-6

Vincent O, Kuchin S, Hong SP, Townley R, Vyas VK, Carlson M

Abstract

Sip4 is a Zn(2)Cys(6) transcriptional activator that binds to the carbon source-responsive elements of gluconeogenic genes in Saccharomyces cerevisiae. The Snf1 protein kinase interacts with Sip4 and regulates its phosphorylation and activator function in response to glucose limitation; however, evidence suggested that another kinase also regulates Sip4. Here we examine the role of the Srb10 kinase, a component of the RNA polymerase II holoenzyme that has been primarily implicated in transcriptional repression but also positively regulates Gal4. We show that Srb10 is required for phosphorylation of Sip4 during growth in nonfermentable carbon sources and that the catalytic activity of Srb10 stimulates the ability of LexA-Sip4 to activate transcription of a reporter. Srb10 and Sip4 coimmunoprecipitate from cell extracts and interact in two-hybrid assays, suggesting that Srb10 regulates Sip4 directly. We also present evidence that the Srb10 and Snf1 kinases interact with different regions of Sip4. These findings support the view that the Srb10 kinase not only plays negative roles in transcriptional control but also has broad positive roles during growth in carbon sources other than glucose.

MeSH Terms
Bacterial Proteins/genetics,metabolism Basic-Leucine Zipper Transcription Factors Cyclin-Dependent Kinase 8 Cyclin-Dependent Kinases/genetics,metabolism Fungal Proteins/metabolism Glucose/metabolism Mutagenesis Phosphorylation Precipitin Tests Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Serine Endopeptidases/genetics,metabolism Trans-Activators/genetics,metabolism Transcriptional Activation Two-Hybrid System Techniques Zinc Fingers
Chemicals
Bacterial Proteins Basic-Leucine Zipper Transcription Factors CAT8 protein, S cerevisiae Fungal Proteins LexA protein, Bacteria Recombinant Fusion Proteins SIP4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Cyclin-Dependent Kinase 8 Cyclin-Dependent Kinases SSN3 protein, S cerevisiae Serine Endopeptidases Glucose
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vincent O
Department of Genetics and Development, Columbia University, 701 W. 168th Street, HSC 922, New York, NY 10032, USA.
Kuchin S
Hong S P
Townley R
Vyas V K
Carlson M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.17.5790-5796.2001
Published
2001-09-00
Pages
5790-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87298
Subset
IM
Grants
NIGMS NIH HHS · R37 GM034095 · United States
NIGMS NIH HHS · R01 GM047259 · United States
NIGMS NIH HHS · GM34095 · United States
NIGMS NIH HHS · S06 GM008224 · United States
NIGMS NIH HHS · GM47259 · United States
NIGMS NIH HHS · GM08224 · United States
NIGMS NIH HHS · R01 GM034095 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.17.5790-5796.2001 PMC Full Text View journal details
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