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PMID: 11483732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Influenza virus ns1 protein induces apoptosis in cultured cells.

Journal of virology ·Vol. 75 ·No. 17 ·2001-09-00 ·Pages 7875-81

Schultz-Cherry S, Dybdahl-Sissoko N, Neumann G, Kawaoka Y, Hinshaw VS

Abstract

The importance of influenza viruses as worldwide pathogens in humans, domestic animals, and poultry is well recognized. Discerning how influenza viruses interact with the host at a cellular level is crucial for a better understanding of viral pathogenesis. Influenza viruses induce apoptosis through mechanisms involving the interplay of cellular and viral factors that may depend on the cell type. However, it is unclear which viral genes induce apoptosis. In these studies, we show that the expression of the nonstructural (NS) gene of influenza A virus is sufficient to induce apoptosis in MDCK and HeLa cells. Further studies showed that the multimerization domain of the NS1 protein but not the effector domain is required for apoptosis. However, this mutation is not sufficient to inhibit apoptosis using whole virus.

MeSH Terms
Animals Apoptosis Cell Line Dogs HeLa Cells Humans Kidney/cytology Mutation Orthomyxoviridae/genetics,physiology Plasmids/genetics Reassortant Viruses/genetics,physiology Transfection Viral Nonstructural Proteins/chemistry,genetics,metabolism
Chemicals
INS1 protein, influenza virus Viral Nonstructural Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schultz-Cherry S
Southeast Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA. sschultz@seprl.usda.gov
Dybdahl-Sissoko N
Neumann G
Kawaoka Y
Hinshaw V S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.75.17.7875-7881.2001
Email
sschultz@seprl.usda.gov
Published
2001-09-00
Pages
7875-81
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC115031
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.75.17.7875-7881.2001 PMC Full Text View journal details
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