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PMID: 8764047 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction cloning of NS1-I, a human protein that binds to the nonstructural NS1 proteins of influenza A and B viruses.

Journal of virology ·Vol. 70 ·No. 8 ·1996-08-00 ·Pages 5363-72

Wolff T, O'Neill RE, Palese P

Abstract

The yeast interaction trap system was used to identify, NS1-I (for NS1 interactor), which is a human protein that binds to the nonstructural NS1 protein of the influenza A virus. NS1-I is a human homolog of the porcine 17beta-estradiol dehydrogenase precursor protein, to which it is 84% identical. We detected only one NS1-I mRNA species, of about 3.0 kb, in HeLa cells, and the NS1-I cDNA was found to have a coding capacity for a 79.6-kDa protein. However, immunoblot analysis detected predominantly a 55-kDa protein in human cells, suggesting that NS1-I, like the porcine 17beta-estradiol dehydrogenase, is posttranslationally processed. Using an in vitro coprecipitation assay, we showed that NS1-I interacts with NS1 proteins from extracts of cells infected with five different influenza A virus strains as well as with the NS1 of an influenza B virus. The fact that influenza A and influenza B virus NS1 proteins bind to NS1-I suggests that this cellular protein plays a role in the influenza virus life cycle.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Estradiol Dehydrogenases/genetics HeLa Cells Humans Influenza A virus/metabolism Influenza B virus/metabolism Influenza, Human/metabolism,virology Molecular Sequence Data Protein Binding Proteins/genetics,metabolism Saccharomyces cerevisiae Sequence Analysis Viral Nonstructural Proteins/metabolism
Chemicals
INS1 protein, influenza virus Proteins Viral Nonstructural Proteins Estradiol Dehydrogenases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wolff T
Department of Microbiology, Mount Sinai School of Medicine, New York 10029, USA.
O'Neill R E
Palese P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.8.5363-5372.1996
Published
1996-08-00
Pages
5363-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190494
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.8.5363-5372.1996 PMC Full Text View journal details
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