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PMID: 11445541 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Division site selection protein DivIVA of Bacillus subtilis has a second distinct function in chromosome segregation during sporulation.

Genes & development ·Vol. 15 ·No. 13 ·2001-07-01 ·Pages 1662-73

Thomaides HB, Freeman M, El Karoui M, Errington J

Abstract

DivIVA is a coiled-coil, tropomyosin-like protein of Gram-positive bacteria. Previous work showed that this protein is targeted to division sites and retained at the cell poles after division. In vegetative cells, DivIVA sequesters the MinCD division inhibitor to the cell poles, thereby helping to direct cell division to the correct midcell site. We now show that DivIVA has a second, quite separate role in sporulating cells of Bacillus subtilis. It again acts at the cell pole but in this case interacts with the chromosome segregation machinery to help position the oriC region of the chromosome at the cell pole, in preparation for polar division. We isolated mutations in divIVA that separate the protein's role in sporulation from its vegetative function in cell division. DivIVA therefore appears to be a bifunctional protein with distinct roles in division-site selection and chromosome segregation.

MeSH Terms
Adenosine Triphosphatases/metabolism Bacillus subtilis/genetics,metabolism,physiology Bacterial Proteins/metabolism Cell Cycle Proteins/genetics,metabolism,physiology Chromosome Segregation/physiology Chromosomes, Bacterial/physiology Escherichia coli Proteins Gene Expression Mutagenesis Sigma Factor/metabolism Spores, Bacterial
Chemicals
Bacterial Proteins Cell Cycle Proteins DivIVA protein, bacteria Escherichia coli Proteins FliA protein, Bacteria Sigma Factor Adenosine Triphosphatases MinD protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thomaides H B
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.
Freeman M
El Karoui M
Errington J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.197501
Published
2001-07-01
Pages
1662-73
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312724
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.197501 PMC Full Text View journal details
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