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PMID: 9573042 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of a prokaryotic SMC protein involved in chromosome partitioning.

Genes & development ·Vol. 12 ·No. 9 ·1998-05-01 ·Pages 1254-9

Britton RA, Lin DC, Grossman AD

Abstract

smc of Bacillus subtilis encodes a homolog of eukaryotic SMC proteins involved in chromosome condensation, pairing, and partitioning. A null mutation in B. subtilis smc caused a temperature-sensitive-lethal phenotype in rich medium. Under permissive conditions, the mutant had abnormal nucleoids, approximately 10% of the cells were anucleate, and assembly of foci of the chromosome partitioning protein Spo0J was altered. In combination with a null mutation in spo0J, the smc mutation caused a synthetic phenotype; cell growth was slower and approximately 25% of the cells were anucleate. Our results demonstrate that the B. subtilis Smc protein, like its eukaryotic counterpart, plays an important role in chromosome structure and partitioning.

MeSH Terms
Bacillus subtilis/cytology,genetics,metabolism Bacterial Proteins/genetics,metabolism Cell Division/genetics Chromosomes, Bacterial Genes, Bacterial Models, Biological Mutation Phenotype Recombinant Fusion Proteins/genetics,metabolism Spores, Bacterial/genetics Subcellular Fractions/metabolism Temperature
Chemicals
Bacterial Proteins Recombinant Fusion Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Britton R A
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Lin D C
Grossman A D
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.9.1254
Published
1998-05-01
Pages
1254-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316777
Subset
IM
Grants
NIGMS NIH HHS · GM41934 · United States
External Links
PubMed source DOI: 10.1101/gad.12.9.1254 PMC Full Text View journal details
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