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PMID: 10515933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

MinDE-dependent pole-to-pole oscillation of division inhibitor MinC in Escherichia coli.

Journal of bacteriology ·Vol. 181 ·No. 20 ·1999-10-00 ·Pages 6419-24

Raskin DM, de Boer PA

Abstract

By inhibiting FtsZ ring formation near the cell ends, the MinC protein plays a critical role in proper positioning of the division apparatus in Escherichia coli. MinC activity requires that of MinD, and the MinE peptide provides topological specificity by suppressing MinC-MinD-mediated division inhibition specifically at the middle of the cell. We recently presented evidence that MinE not only accumulates in an FtsZ-independent ring structure at the cell's middle but also imposes a unique dynamic localization pattern upon MinD in which the latter accumulates alternately in either one of the cell halves in what appears to be a rapidly oscillating membrane association-dissociation cycle. Here we show that functional green fluorescent protein-MinC displays a very similar oscillatory behavior which is dependent on both MinD and MinE and independent of FtsZ. The results support a model in which MinD recruits MinC to its site of action and in which FtsZ ring assembly at each of the cell ends is blocked in an intermittent and alternate fashion.

MeSH Terms
Adenosine Triphosphatases/metabolism Bacterial Proteins/genetics,isolation & purification,metabolism Cell Compartmentation Cell Cycle Proteins Cell Division Cytoskeletal Proteins Escherichia coli/cytology Escherichia coli Proteins Green Fluorescent Proteins Growth Inhibitors/metabolism Luminescent Proteins/genetics,isolation & purification Models, Biological Periodicity Recombinant Fusion Proteins/isolation & purification
Chemicals
Bacterial Proteins Cell Cycle Proteins Cytoskeletal Proteins Escherichia coli Proteins FtsZ protein, Bacteria Growth Inhibitors Luminescent Proteins MinC protein, Bacteria MinE protein, E coli Recombinant Fusion Proteins Green Fluorescent Proteins Adenosine Triphosphatases MinD protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Raskin D M
Department of Molecular Biology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4960, USA.
de Boer P A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.20.6419-6424.1999
Published
1999-10-00
Pages
6419-24
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC103778
Subset
IM
Grants
NIGMS NIH HHS · R01 GM057059 · United States
NIGMS NIH HHS · T32 GM008056 · United States
NIGMS NIH HHS · GM-57059 · United States
NIGMS NIH HHS · T32GM08056 · United States
External Links
PubMed source DOI: 10.1128/JB.181.20.6419-6424.1999 PMC Full Text View journal details
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