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PMID: 11427545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections.

Journal of clinical microbiology ·Vol. 39 ·No. 7 ·2001-07-00 ·Pages 2397-404

Araki K, Yoshimatsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J

Abstract

Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Viral/immunology Enzyme-Linked Immunosorbent Assay Hantaan virus/classification Hantavirus/classification,metabolism Hantavirus Infections/diagnosis Hemorrhagic Fever with Renal Syndrome/diagnosis Humans Immune Sera/immunology Nucleocapsid Proteins/genetics,immunology,metabolism Nucleopolyhedroviruses/genetics,metabolism Rabbits Recombinant Proteins/genetics,immunology,metabolism Serotyping
Chemicals
Antibodies, Monoclonal Antigens, Viral Immune Sera Nucleocapsid Proteins Recombinant Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Araki K
Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Yoshimatsu K
Ogino M
Ebihara H
Lundkvist A
Kariwa H
Takashima I
Arikawa J
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.39.7.2397-2404.2001
Published
2001-07-00
Pages
2397-404
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC88161
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.39.7.2397-2404.2001 PMC Full Text View journal details
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