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PMID: 9114386 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Puumala hantavirus genome in patients with nephropathia epidemica: correlation of PCR positivity with HLA haplotype and link to viral sequences in local rodents.

Journal of clinical microbiology ·Vol. 35 ·No. 5 ·1997-05-00 ·Pages 1090-6

Plyusnin A, Hörling J, Kanerva M, Mustonen J, Cheng Y, Partanen J, Vapalahti O, Kukkonen SK, Niemimaa J, Henttonen H, Niklasson B, Lundkvist A, Vaheri A

Abstract

Reverse transcription-PCR was used to analyze specimens from 20 Finnish nephropathia epidemica (NE) patients hospitalized during the period from October 1994 to January 1995. Blood and/or urine sediment specimens from seven patients were found to be positive for the genome sequences of Puumala hantavirus (PUU). PCR positivity of the specimens from the patients correlated well with the HLA-DRB1*0301 and HLA B8 alleles, which previously were shown to associate with severe courses of NE. Genetic analysis of the partial M-and/or S-segment sequences obtained from three severely ill NE patients revealed three PUU strains related to but distinct from previously reported strains from Finland. The M-segment sequence of PUU from bank voles trapped near the probable site of infection for one of the patients showed 98.2% identity to that of the PUU strain obtained from the patient, suggesting a link between wild-type PUU from the natural focus and the NE case. The S-segment sequences from the patient and the bank voles, however, showed substantially lower identity (95.8%). As this difference in diversity for M and S genes (1.8 and 4.2%) is atypical for PUU genetic drift, one possibility is that the strain acquired at the putative place of infection is a reassortant one.

MeSH Terms
Adult Aged Animals Female Genome, Viral HLA Antigens/genetics Hantavirus/genetics Hantavirus Infections/immunology,transmission,virology Haplotypes Humans Male Middle Aged Molecular Sequence Data Phylogeny Polymerase Chain Reaction Rodentia Sequence Analysis
Chemicals
HLA Antigens
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Plyusnin A
Department of Virology, Haartman Institute, University of Helsinki, Finland. Alexander.Plyusnin@helsinki.fi
Hörling J
Kanerva M
Mustonen J
Cheng Y
Partanen J
Vapalahti O
Kukkonen S K
Niemimaa J
Henttonen H
Niklasson B
Lundkvist A
Vaheri A
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.35.5.1090-1096.1997
Email
alexander.plyusnin@helsinki.fi
Published
1997-05-00
Pages
1090-6
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC232708
Subset
IM
Databases
GENBANK
L25783, L25784, L36929, L36930, L36943, L36944, M29979, M32750, U22418, U22423, U31534, U35254, U35255, X61034, X61035, Z21497, Z30702, Z48586, Z49098, Z49214, Z55129, Z69985, Z69986, Z69987, Z69988, Z69989, Z69990, Z69991, Z69992, Z70201
External Links
PubMed source DOI: 10.1128/jcm.35.5.1090-1096.1997 PMC Full Text View journal details
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