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PMID: 9705385 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Antigenic characterization of Hantaan and Seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen.

Journal of clinical microbiology ·Vol. 36 ·No. 9 ·1998-09-00 ·Pages 2514-21

Morii M, Yoshimatsu K, Arikawa J, Zhou G, Kariwa H, Takashima I

Abstract

Hantaan virus (HTN) and Seoul virus (SEO) are members of the genus Hantavirus in the family Bunyaviridae and are causative agents of hemorrhagic fever with renal syndrome. The complete and truncated nucleocapsid proteins (NP) of HTN and SEO were expressed by a recombinant baculovirus system. Antigenic characterization of the NP using monoclonal antibodies (MAbs) indicated that the binding sites for the serotype-specific MAbs were located between amino acids (aa) 155 and 429. A Western blot assay indicated that the serotype-specific epitopes were conformation dependent. An indirect immunofluorescence antibody (IFA) assay with the truncated NP (aa 155 to 429) was able to distinguish convalescent-phase sera from HTN and SEO patients. However, the antibody titers with the truncated NP were lower than those with the whole NP. The truncated NP of SEO (aa 155 to 429) could be used as an enzyme-linked immunosorbent assay (ELISA) antigen, but the truncated NP from HTN lost its reactivity when used for ELISA. The IFA assay using baculovirus-expressed truncated NP as an antigen is a rapid, simple, and safe test for distinguishing between HTN and SEO infections by serotype.

MeSH Terms

Adult Animals Antibodies, Monoclonal Cell Line Cloning, Molecular/methods Enzyme-Linked Immunosorbent Assay Epitopes/analysis Female Fluorescent Antibody Technique, Indirect Hantaan virus/classification Hantavirus/classification Hantavirus Infections/blood,diagnosis Hemorrhagic Fever with Renal Syndrome/blood,diagnosis Humans Insecta Male Middle Aged Nucleocapsid Proteins/biosynthesis,chemistry,immunology Nucleopolyhedroviruses Polymerase Chain Reaction/methods Recombinant Proteins/biosynthesis,chemistry,immunology Sequence Deletion Serotyping Transfection

Chemicals

Antibodies, Monoclonal Epitopes Nucleocapsid Proteins Recombinant Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Morii M

Hokkaido University School of Medicine, Hokkaido University, Sapporo 060, Japan.

Yoshimatsu K

Arikawa J

Zhou G

Kariwa H

Takashima I

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.36.9.2514-2521.1998

Published

1998-09-00

Pages

2514-21

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC105155

Subset

External Links

PubMed source DOI: 10.1128/JCM.36.9.2514-2521.1998 PMC Full Text View journal details

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Antigenic characterization of Hantaan and Seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen.

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