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PMID: 113418 Published · ppublish English Journal Article

Increase of gap junctions between pancreatic B-cells during stimulation of insulin secretion.

The Journal of cell biology ·Vol. 82 ·No. 2 ·1979-08-00 ·Pages 441-48

Meda P, Perrelet A, Orci L

Abstract

The development of gap junctions between pancreatic B-cells was quantitatively assessed in freeze-fracture replicas of isolated rat islets under different conditions of insulin secretion. The results show that in resting B-cells, gap junctions are small and scarce but that these junctions increase when insulin secretion is stimulated. Both a short (90 min) stimulation by glucose in vitro and a prolonged (2.5 d) stimulation by glibenclamide in vivo raise the number of gap junctions; in addition, the glibenclamide stimulation causes an increase in the size of individual gap junctions. As a consequence, the total area occupied by gap junctions on the B-cell membrane and the ratio of this area to the cell volume were found significantly increased in the latter condition. The slight increase of these values observed after the glucose stimulation did not reach significance. These data indicate a change of gap junctions during the secretory activity of the pancreatic B-cells. The possibility that the coupling of the cells is affected by the treatment is discussed.

MeSH Terms
Animals Cell Count Female Freeze Fracturing Glucose/pharmacology Glyburide/pharmacology Insulin/metabolism Insulin Secretion Intercellular Junctions/ultrastructure Islets of Langerhans/drug effects,metabolism,ultrastructure Rats
Chemicals
Insulin Glucose Glyburide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meda P
Perrelet A
Orci L
References (33)
33 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.82.2.441
Published
1979-08-00
Pages
441-48
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110458
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.82.2.441 PMC Full Text View journal details
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