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PMID: 11244079 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A mutation in the 5' untranslated region increases stability of norA mRNA, encoding a multidrug resistance transporter of Staphylococcus aureus.

Journal of bacteriology ·Vol. 183 ·No. 7 ·2001-04-00 ·Pages 2367-71

Fournier B, Truong-Bolduc QC, Zhang X, Hooper DC

Abstract

NorA, a multidrug efflux pump in Staphylococcus aureus, protects the cell from multiple drugs, including quinolones. The flqB mutation (T-->G) in the 5' untranslated region upstream of norA causes norA overexpression of 4.9-fold in cis, as measured in norA::blaZ fusions. The transcriptional initiation site of norA was unchanged in mutant and wild-type strains, but the half-life of norA mRNA was increased 4.8-fold in the flqB mutant compared to the wild-type strain. Computer-generated folding of the first 68 nucleotides of the norA transcript predicts an additional stem-loop and changes in a putative RNase III cleavage site in the flqB mutant.

MeSH Terms
5' Untranslated Regions Bacterial Proteins/chemistry,genetics Base Sequence Molecular Sequence Data Multidrug Resistance-Associated Proteins Mutation RNA, Messenger/chemistry Staphylococcus aureus/genetics
Chemicals
5' Untranslated Regions Bacterial Proteins Multidrug Resistance-Associated Proteins RNA, Messenger NorA protein, Staphylococcus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fournier B
Infectious Disease Division and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114-2696, USA.
Truong-Bolduc Q C
Zhang X
Hooper D C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.7.2367-2371.2001
Published
2001-04-00
Pages
2367-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95146
Subset
IM
Grants
NIDDK NIH HHS · P30 DK043351 · United States
NIDDK NIH HHS · P30 DK-43351 · United States
NIAID NIH HHS · R01 AI023988 · United States
NIAID NIH HHS · R37 AI023988 · United States
NIAID NIH HHS · AI23988 · United States
External Links
PubMed source DOI: 10.1128/JB.183.7.2367-2371.2001 PMC Full Text View journal details
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