Home LiteratureArticle Details
PMID: 10633102 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Different processing of an mRNA species in Bacillus subtilis and Escherichia coli.

Journal of bacteriology ·Vol. 182 ·No. 3 ·2000-02-00 ·Pages 689-95

Persson M, Glatz E, Rutberg B

Abstract

Expression of the Bacillus subtilis glpD gene, which encodes glycerol-3-phosphate (G3P) dehydrogenase, is controlled by termination or antitermination of transcription. The untranslated leader sequence of glpD contains an inverted repeat that gives rise to a transcription terminator. In the presence of G3P, the antiterminator protein GlpP binds to glpD leader mRNA and promotes readthrough of the terminator. Certain mutations in the inverted repeat of the glpD leader result in GlpP-independent, temperature-sensitive (TS) expression of glpD. The TS phenotype is due to temperature-dependent degradation of the glpD mRNA. In the presence of GlpP, the glpD mRNA is stabilized. glpD leader-lacZ fusions were integrated into the chromosomes of B. subtilis and Escherichia coli. Determination of steady-state levels of fusion mRNA in B. subtilis showed that the stability of the fusion mRNA is determined by the glpD leader part. Comparison of steady-state levels and half-lives of glpD leader-lacZ fusion mRNA in B. subtilis and E. coli revealed significant differences. A glpD leader-lacZ fusion transcript that was unstable in B. subtilis was considerably more stable in E. coli. GlpP, which stabilizes the transcript in B. subtilis, did not affect its stability in E. coli. Primer extension analysis showed that the glpD leader-lacZ fusion transcript is processed differently in B. subtilis and in E. coli. The dominating cleavage site in E. coli was barely detectable in B. subtilis. This site was shown to be a target of E. coli RNase III.

MeSH Terms
5' Untranslated Regions Bacillus subtilis/genetics Base Sequence Blotting, Northern Escherichia coli/genetics Glycerolphosphate Dehydrogenase/genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Messenger/metabolism Transcription, Genetic
Chemicals
5' Untranslated Regions RNA, Messenger Glycerolphosphate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Persson M
Department of Microbiology, Lund University, Sölvegatan 12, S-223 62 Lund, Sweden. martin.persson@mikrbiol.lu.se
Glatz E
Rutberg B
References (39)
39 references, click to expand
  1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  2. Enzymatic basis for hydrolytic versus phosphorolytic mRNA degradation in Escherichia coli and Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3277-80 PMID: 1707536
  3. Expression of the gene encoding glycerol-3-phosphate dehydrogenase (glpD) in Bacillus subtilis is controlled by antitermination.
    Mol Microbiol. 1991 Dec;5(12):2891-900 PMID: 1809833
  4. Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
    J Biol Chem. 1998 Jul 31;273(31):19542-7 PMID: 9677377
  5. Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci.
    J Bacteriol. 1997 Dec;179(23):7379-85 PMID: 9393702
  6. Antitermination of transcription of catabolic operons.
    Mol Microbiol. 1997 Feb;23(3):413-21 PMID: 9044276
  7. Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA.
    Microbiology. 1998 Feb;144 ( Pt 2):449-56 PMID: 9493382
  8. An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription.
    Mol Microbiol. 1992 Oct;6(20):2931-8 PMID: 1479885
  9. mRNA degradation. A tale of poly(A) and multiprotein machines.
    Trends Genet. 1999 Jan;15(1):24-8 PMID: 10087930
  10. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  11. A dual role for the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability.
    Mol Microbiol. 1996 Jan;19(2):319-28 PMID: 8825777
  12. A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli.
    Genes Dev. 1992 Jan;6(1):135-48 PMID: 1370426
  13. Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1986 Jan;83(1):120-4 PMID: 2417233
  14. Degradation of mRNA in Escherichia coli: an old problem with some new twists.
    Prog Nucleic Acid Res Mol Biol. 1999;62:55-108 PMID: 9932452
  15. Changes in the stability of specific mRNA species in response to growth stage in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1990 Nov;87(21):8355-9 PMID: 1700430
  16. Substrate specificity of an RNase III-like activity from Bacillus subtilis.
    J Biol Chem. 1994 Dec 16;269(50):31450-6 PMID: 7527387
  17. The C-terminal half of RNase E, which organizes the Escherichia coli degradosome, participates in mRNA degradation but not rRNA processing in vivo.
    Mol Microbiol. 1999 Jul;33(1):188-99 PMID: 10411735
  18. A series of integrative plasmids for Bacillus subtilis containing unique cloning sites in all three open reading frames for translational lacZ fusions.
    Gene. 1994 Jul 22;145(1):151-2 PMID: 7519159
  19. The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site.
    J Mol Biol. 1996 Jan 26;255(3):349-55 PMID: 8568879
  20. Analysis of mRNA decay and rRNA processing in Escherichia coli multiple mutants carrying a deletion in RNase III.
    J Bacteriol. 1993 Jan;175(1):229-39 PMID: 8416898
  21. The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly.
    Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11637-42 PMID: 9751718
  22. In the absence of translation, RNase E can bypass 5' mRNA stabilizers in Escherichia coli.
    J Mol Biol. 1998 Sep 18;282(2):241-54 PMID: 9735284
  23. RNase E: still a wonderfully mysterious enzyme.
    Mol Microbiol. 1997 Mar;23(6):1099-106 PMID: 9106202
  24. The importance of the 5'-region in regulating the stability of sdh mRNA in Bacillus subtilis.
    Mol Microbiol. 1990 Nov;4(11):1881-9 PMID: 1707123
  25. Initiation of mRNA decay in Bacillus subtilis.
    Mol Microbiol. 1993 Mar;7(5):705-17 PMID: 7682278
  26. Modulation of the activity of RNase E in vitro by RNA sequences and secondary structures 5' to cleavage sites.
    J Biol Chem. 1997 Jan 3;272(1):609-16 PMID: 8995304
  27. The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg.
    Mol Gen Genet. 1987 Sep;209(2):335-42 PMID: 2823077
  28. On finding all suboptimal foldings of an RNA molecule.
    Science. 1989 Apr 7;244(4900):48-52 PMID: 2468181
  29. The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
    Nature. 1997 Nov 20;390(6657):249-56 PMID: 9384377
  30. Simian virus 40 major late promoter: a novel tripartite structure that includes intragenic sequences.
    Mol Cell Biol. 1988 May;8(5):2021-33 PMID: 2838741
  31. A polypurine sequence that acts as a 5' mRNA stabilizer in Bacillus subtilis.
    J Bacteriol. 1995 Jun;177(12):3465-71 PMID: 7539420
  32. Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes.
    J Bacteriol. 1996 Nov;178(22):6443-50 PMID: 8932299
  33. Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability.
    Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14118-23 PMID: 9826663
  34. The global regulator CsrA of Escherichia coli is a specific mRNA-binding protein.
    J Bacteriol. 1997 Jul;179(14):4639-42 PMID: 9226279
  35. The Bacillus subtilis glpD leader and antiterminator protein GlpP provide a target for glucose repression in Escherichia coli.
    FEMS Microbiol Lett. 1998 May 1;162(1):93-6 PMID: 9595668
  36. The ompA 5' untranslated region impedes a major pathway for mRNA degradation in Escherichia coli.
    Mol Microbiol. 1994 Jun;12(5):707-16 PMID: 8052123
  37. The decay of bacterial messenger RNA.
    Prog Nucleic Acid Res Mol Biol. 1996;52:153-216 PMID: 8821261
  38. Properties of a Bacillus subtilis polynucleotide phosphorylase deletion strain.
    J Bacteriol. 1996 Apr;178(8):2375-82 PMID: 8636041
  39. Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli.
    J Bacteriol. 1975 Oct;124(1):307-16 PMID: 1100605
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.3.689-695.2000
Email
martin.persson@mikrbiol.lu.se
Published
2000-02-00
Pages
689-95
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94331
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.3.689-695.2000 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2