Home LiteratureArticle Details
PMID: 11238378 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem.

Genes & development ·Vol. 15 ·No. 5 ·2001-03-01 ·Pages 581-90

Sakamoto T, Kamiya N, Ueguchi-Tanaka M, Iwahori S, Matsuoka M

Abstract

To identify genes targeted by the tobacco KNOX homeodomain protein, Nicotiana tabacum homeobox 15 (NTH15), we have generated an inducible system using the human glucocorticoid receptor. In this system, steroid treatment strictly induced NTH15 function and immediately suppressed the expression of a gibberellin (GA) biosynthetic gene encoding GA 20-oxidase (Ntc12) and also resulted in a decrease in bioactive GA levels. The repression of Ntc12 was observed even when indirect effects were blocked by cycloheximide. NTH15 mRNA was present in corpus cells of the shoot apical meristem (SAM), whereas Ntc12 mRNA was observed in leaf primordia and rib meristem but not in the corpus. Recombinant NTH15 protein strongly bound to a 5-bp dyadsymmetric sequence, GTGAC, in the first intron of Ntc12 in vitro. Mutation of this sequence in the Ntc12 gene abolished the NTH15-dependent suppression of Ntc12 in the corpus of the SAM. Our results indicate that NTH15 directly represses Ntc12 expression in the corpus of the wild-type SAM to maintain the indeterminate state of corpus cells. The suppression of NTH15 within cells at the flanks of the SAM permits GA biosynthesis, which promotes organized cell proliferation and consequently induces the determination of cell fate.

MeSH Terms
Cell Division Gene Expression Regulation, Plant Gibberellins/biosynthesis,genetics Homeodomain Proteins/genetics,metabolism Humans In Situ Hybridization Introns Meristem/cytology,genetics,metabolism Mixed Function Oxygenases/genetics,metabolism Plant Proteins Plants, Genetically Modified Plants, Toxic Receptors, Glucocorticoid/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Tobacco/cytology,genetics,metabolism
Chemicals
Gibberellins Homeodomain Proteins Kn1 protein, plant NTH15 protein, Nicotiana tabacum Plant Proteins Receptors, Glucocorticoid Recombinant Fusion Proteins Mixed Function Oxygenases gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sakamoto T
Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Kamiya N
Ueguchi-Tanaka M
Iwahori S
Matsuoka M
References (35)
35 references, click to expand
  1. Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1.
    Plant Cell Physiol. 1997 May;38(5):638-42 PMID: 9210336
  2. GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their Regulation.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:431-460 PMID: 15012270
  3. Loss-of-function mutations in the maize homeobox gene, knotted1, are defective in shoot meristem maintenance.
    Development. 1997 Aug;124(16):3045-54 PMID: 9272946
  4. A steroid-inducible gene expression system for plant cells.
    Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10421-5 PMID: 1961707
  5. Expression of a rice homeobox gene causes altered morphology of transgenic plants.
    Plant Cell. 1993 Sep;5(9):1039-48 PMID: 8104574
  6. Ectopic expression of the Arabidopsis transcriptional activator Athb-1 alters leaf cell fate in tobacco.
    Plant Cell. 1995 Nov;7(11):1773-85 PMID: 8535134
  7. Are homeobox knotted-like genes and cytokinins the leaf architects?
    Plant Physiol. 1999 Feb;119(2):371-4 PMID: 9952431
  8. A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif.
    J Biol Chem. 1995 Dec 29;270(52):31178-88 PMID: 8537382
  9. Ectopic expression of maize knotted1 results in the cytokinin-autotrophic growth of cultured tobacco tissues.
    Planta. 2000 May;210(6):884-9 PMID: 10872218
  10. Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator.
    Science. 1994 Oct 21;266(5184):436-9 PMID: 7939683
  11. Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development.
    Mol Gen Genet. 1993 Apr;238(1-2):106-19 PMID: 7683079
  12. Over-expression of tobacco knotted1-type class1 homeobox genes alters various leaf morphology.
    Plant Cell Physiol. 2000 May;41(5):583-90 PMID: 10929941
  13. A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis.
    Nature. 1996 Jan 4;379(6560):66-9 PMID: 8538741
  14. Leaf senescence is delayed in tobacco plants expressing the maize homeobox gene knotted1 under the control of a senescence-activated promoter.
    Plant Cell. 1999 Jun;11(6):1073-80 PMID: 10368178
  15. Abnormal cell divisions in leaf primordia caused by the expression of the rice homeobox gene OSH1 lead to altered morphology of leaves in transgenic tobacco.
    Mol Gen Genet. 1996 Apr 24;251(1):13-22 PMID: 8628242
  16. Ectopic expression of a tobacco homeobox gene, NTH15, dramatically alters leaf morphology and hormone levels in transgenic tobacco.
    Plant Cell Physiol. 1997 Aug;38(8):917-27 PMID: 9327591
  17. LAM-1 and FAT Genes Control Development of the Leaf Blade in Nicotiana sylvestris.
    Plant Cell. 1993 Sep;5(9):1029-1038 PMID: 12271096
  18. The conserved KNOX domain mediates specificity of tobacco KNOTTED1-type homeodomain proteins.
    Plant Cell. 1999 Aug;11(8):1419-32 PMID: 10449577
  19. Alteration of hormone levels in transgenic tobacco plants overexpressing the rice homeobox gene OSH1.
    Plant Physiol. 1998 Feb;116(2):471-6 PMID: 9489007
  20. Phytochrome activation of two nuclear genes requires cytoplasmic protein synthesis.
    EMBO J. 1989 Oct;8(10):2777-83 PMID: 2583082
  21. KNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis.
    Plant Cell. 1996 Aug;8(8):1277-89 PMID: 8776897
  22. DNA binding sites recognised in vitro by a knotted class 1 homeodomain protein encoded by the hooded gene, k, in barley (Hordeum vulgare).
    FEBS Lett. 1997 May 12;408(1):25-9 PMID: 9180261
  23. A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants.
    Plant Cell. 1994 Dec;6(12):1859-76 PMID: 7866029
  24. Regional expression of the rice KN1-type homeobox gene family during embryo, shoot, and flower development.
    Plant Cell. 1999 Sep;11(9):1651-64 PMID: 10488233
  25. Expression of photosynthetic genes from the C4 plant, maize, in tobacco.
    Mol Gen Genet. 1991 Mar;225(3):411-9 PMID: 1850086
  26. Knots in the family tree: evolutionary relationships and functions of knox homeobox genes.
    Plant Mol Biol. 2000 Jan;42(1):151-66 PMID: 10688134
  27. Overexpression of the maize homeo box gene, KNOTTED-1, causes a switch from determinate to indeterminate cell fates.
    Genes Dev. 1993 May;7(5):787-95 PMID: 7684007
  28. A glucocorticoid-mediated transcriptional induction system in transgenic plants.
    Plant J. 1997 Mar;11(3):605-12 PMID: 9107046
  29. Over-expression of a tobacco homeobox gene, NTH15, decreases the expression of a gibberellin biosynthetic gene encoding GA 20-oxidase.
    Plant J. 1998 Aug;15(3):391-400 PMID: 9750350
  30. Decreased GA1 content caused by the overexpression of OSH1 is accompanied by suppression of GA 20-oxidase gene expression.
    Plant Physiol. 1998 Aug;117(4):1179-84 PMID: 9701574
  31. The expression of tobacco knotted1-type class 1 homeobox genes correspond to regions predicted by the cytohistological zonation model.
    Plant J. 1999 May;18(4):337-47 PMID: 10406119
  32. A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3/PISTILLATA.
    Cell. 1998 Jan 9;92(1):93-103 PMID: 9489703
  33. Activation of floral meristem identity genes in Arabidopsis.
    Nature. 1996 Nov 7;384(6604):59-62 PMID: 8900276
  34. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  35. Primary structure and expression of a functional human glucocorticoid receptor cDNA.
    Nature. 1985 Dec 19-1986 Jan 1;318(6047):635-41 PMID: 2867473
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.867901
Published
2001-03-01
Pages
581-90
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312643
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.867901 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2