Home LiteratureArticle Details
PMID: 11159397 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular identification of a mechanosensitive channel in archaea.

Biophysical journal ·Vol. 80 ·No. 1 ·2001-01-00 ·Pages 229-40

Kloda A, Martinac B

Abstract

The TM1 domain of the large conductance mechanosensitive (MS) channel of Escherichia coli was used as a genetic probe to search the genomic database of the archaeon Methanoccoccus jannashii for MscL homologs. We report that the hypothetical protein MJ0170 of M. jannashii exhibited 38.5% sequence identity with the TM1 domain of Eco-MscL. Moreover, MJ0170 was found to be a conserved homolog of MscS, the second type of E. coli MS channel encoded by the yggB gene. Furthermore, we identified a cluster of charged residues KIKEE in the C-terminus of MJ0170 that strikingly resembled the charged C-terminal amino acid cluster present in Eco-MscL (RKKEE). We cloned and expressed MJ0170 in E. coli, which when reconstituted into liposomes or expressed in the cell membrane of giant E. coli spheroplasts, exhibited similar activity to the bacterial MS channels. Our study suggests that the M. jannashii MS channel and its homologs evolved as a result of gene duplication of the ancestral MscL-like molecule with the TM1 domain remaining the most conserved structural motif among prokaryotic MS channels.

MeSH Terms
Amino Acid Sequence Archaea/genetics,physiology Archaeal Proteins/chemistry,genetics,physiology Bacterial Proteins/chemistry,genetics,physiology Biophysical Phenomena Biophysics Conserved Sequence Escherichia coli/genetics,growth & development,physiology Escherichia coli Proteins Evolution, Molecular Ion Channels/chemistry,genetics,physiology Methanococcus/genetics,physiology Molecular Sequence Data Phylogeny Protein Structure, Secondary Protein Structure, Tertiary Recombinant Proteins/chemistry,genetics Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Archaeal Proteins Bacterial Proteins Escherichia coli Proteins Ion Channels MscL protein, E coli Recombinant Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kloda A
Department of Pharmacology, QEII Medical Center, The University of Western Australia, Nedlands WA 6907, Australia.
Martinac B
References (39)
39 references, click to expand
  1. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone.
    Nature. 1994 Mar 17;368(6468):265-8 PMID: 7511799
  2. Molecular dissection of the large mechanosensitive ion channel (MscL) of E. coli: mutants with altered channel gating and pressure sensitivity.
    J Membr Biol. 1997 May 1;157(1):17-25 PMID: 9141355
  3. Molecular evolution. Archaea and eukaryotes grow closer.
    Science. 1994 May 27;264(5163):1251 PMID: 8191278
  4. Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli.
    J Biol Chem. 1995 Aug 4;270(31):18329-34 PMID: 7543101
  5. Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli.
    Biophys J. 1997 Oct;73(4):1925-31 PMID: 9336188
  6. Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress.
    J Biol Chem. 1997 Dec 19;272(51):32150-7 PMID: 9405414
  7. Homology modelling of two subtilisin-like proteases from the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus stetteri.
    Protein Eng. 1997 Aug;10(8):905-14 PMID: 9415440
  8. Mechanosensitive ion channels of the archaeon Haloferax volcanii.
    J Biol Chem. 1998 May 15;273(20):12116-9 PMID: 9575156
  9. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.
    Mol Microbiol. 1998 May;28(3):583-92 PMID: 9632260
  10. A mammalian two pore domain mechano-gated S-like K+ channel.
    EMBO J. 1998 Aug 3;17(15):4283-90 PMID: 9687497
  11. One face of a transmembrane helix is crucial in mechanosensitive channel gating.
    Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11471-5 PMID: 9736761
  12. Release of thioredoxin via the mechanosensitive channel MscL during osmotic downshock of Escherichia coli cells.
    J Biol Chem. 1998 Oct 9;273(41):26670-4 PMID: 9756908
  13. Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.
    Science. 1998 Dec 18;282(5397):2220-6 PMID: 9856938
  14. Mechanosensitive channels of bacteria.
    Methods Enzymol. 1999;294:458-82 PMID: 9916243
  15. Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL.
    J Gen Physiol. 1999 Apr;113(4):525-40 PMID: 10102934
  16. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity.
    EMBO J. 1999 Apr 1;18(7):1730-7 PMID: 10202137
  17. Phylogenetic classification and the universal tree.
    Science. 1999 Jun 25;284(5423):2124-9 PMID: 10381871
  18. 'Feeling the pressure': structural insights into a gated mechanosensitive channel.
    Curr Opin Struct Biol. 1999 Aug;9(4):448-54 PMID: 10449367
  19. Structure and function of the bacterial mechanosensitive channel of large conductance.
    Protein Sci. 1999 Oct;8(10):1915-21 PMID: 10548036
  20. Contributions of the different extramembranous domains of the mechanosensitive ion channel MscL to its response to membrane tension.
    J Biol Chem. 2000 Jan 14;275(2):1015-22 PMID: 10625640
  21. Mechanically gated channel activity in cytoskeleton-deficient plasma membrane blebs and vesicles from Xenopus oocytes.
    J Physiol. 2000 Feb 15;523 Pt 1:117-30 PMID: 10673548
  22. Pharmacological modifications of the sodium channels of frog nerve.
    J Gen Physiol. 1968 Feb;51(2):199-219 PMID: 5641635
  23. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
    Pflugers Arch. 1981 Aug;391(2):85-100 PMID: 6270629
  24. Pressure-sensitive ion channel in Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2297-301 PMID: 2436228
  25. Mechanoelectrical transduction by hair cells.
    Annu Rev Biophys Biophys Chem. 1988;17:99-124 PMID: 3293600
  26. Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels.
    Biophys J. 1989 Sep;56(3):631-6 PMID: 2477074
  27. Mechanosensitive ion channels of E. coli activated by amphipaths.
    Nature. 1990 Nov 15;348(6298):261-3 PMID: 1700306
  28. Molecular evolution of voltage-sensitive ion channel genes: on the origins of electrical excitability.
    Mol Biol Evol. 1993 Jan;10(1):221-42 PMID: 7680747
  29. Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution.
    Biophys J. 1993 Jul;65(1):177-83 PMID: 7690260
  30. Archaeal ubiquity.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6228-30 PMID: 8692796
  31. Multiple mechanosensitive ion channels from Escherichia coli, activated at different thresholds of applied pressure.
    J Membr Biol. 1996 May;151(2):175-87 PMID: 8661505
  32. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.
    Science. 1996 Aug 23;273(5278):1058-73 PMID: 8688087
  33. Life's last domain.
    Science. 1996 Aug 23;273(5278):1043-5 PMID: 8711482
  34. The pharmacology of mechanogated membrane ion channels.
    Pharmacol Rev. 1996 Jun;48(2):231-52 PMID: 8804105
  35. Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli.
    Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11652-7 PMID: 8876191
  36. The molecules of mechanosensation.
    Annu Rev Neurosci. 1997;20:567-94 PMID: 9056725
  37. Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities.
    Annu Rev Physiol. 1997;59:633-57 PMID: 9074781
  38. A molecular view of microbial diversity and the biosphere.
    Science. 1997 May 2;276(5313):734-40 PMID: 9115194
  39. There must be a prokaryote somewhere: microbiology's search for itself.
    Microbiol Rev. 1994 Mar;58(1):1-9 PMID: 8177167
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(01)76009-2
Published
2001-01-00
Pages
229-40
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1301228
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(01)76009-2 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2