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PMID: 9687497 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mammalian two pore domain mechano-gated S-like K+ channel.

The EMBO journal ·Vol. 17 ·No. 15 ·1998-08-03 ·Pages 4283-90

Patel AJ, Honoré E, Maingret F, Lesage F, Fink M, Duprat F, Lazdunski M

Abstract

Aplysia S-type K+ channels of sensory neurons play a dominant role in presynaptic facilitation and behavioural sensitization. They are closed by serotonin via cAMP-dependent phosphorylation, whereas they are opened by arachidonic acid, volatile general anaesthetics and mechanical stimulation. We have identified a cloned mammalian two P domain K+ channel sharing the properties of the S channel. In addition, the recombinant channel is opened by lipid bilayer amphipathic crenators, while it is closed by cup-formers. The cytoplasmic C-terminus contains a charged region critical for chemical and mechanical activation, as well as a phosphorylation site required for cAMP inhibition.

MeSH Terms
Amino Acid Sequence Animals Aplysia Arachidonic Acid/pharmacology COS Cells Cyclic AMP-Dependent Protein Kinases/metabolism Humans Invertebrate Hormones/chemistry,physiology Ion Channel Gating/drug effects,physiology Molecular Sequence Data Phosphorylation Potassium Channels/chemistry,drug effects,metabolism,physiology Potassium Channels, Tandem Pore Domain Protein Structure, Tertiary Shab Potassium Channels
Chemicals
Invertebrate Hormones KCNK1 protein, human Potassium Channels Potassium Channels, Tandem Pore Domain Shab Potassium Channels Shab protein, Aplysia potassium channel protein TREK-1 Arachidonic Acid Cyclic AMP-Dependent Protein Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Patel A J
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, Valbonne, France.
Honoré E
Maingret F
Lesage F
Fink M
Duprat F
Lazdunski M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.15.4283
Published
1998-08-03
Pages
4283-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170762
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.15.4283 PMC Full Text View journal details
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