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PMID: 11073988 Published · ppublish English Comparative Study Journal Article

A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex.

Molecular and cellular biology ·Vol. 20 ·No. 23 ·2000-12-00 ·Pages 8879-88

Nie Z, Xue Y, Yang D, Zhou S, Deroo BJ, Archer TK, Wang W

Abstract

The SWI/SNF family of chromatin-remodeling complexes facilitates gene activation by assisting transcription machinery to gain access to targets in chromatin. This family includes BAF (also called hSWI/SNF-A) and PBAF (hSWI/SNF-B) from humans and SWI/SNF and Rsc from Saccharomyces cerevisiae. However, the relationship between the human and yeast complexes is unclear because all human subunits published to date are similar to those of both yeast SWI/SNF and Rsc. Also, the two human complexes have many identical subunits, making it difficult to distinguish their structures or functions. Here we describe the cloning and characterization of BAF250, a subunit present in human BAF but not PBAF. BAF250 contains structural motifs conserved in yeast SWI1 but not in any Rsc components, suggesting that BAF is related to SWI/SNF. BAF250 is also a homolog of the Drosophila melanogaster Osa protein, which has been shown to interact with a SWI/SNF-like complex in flies. BAF250 possesses at least two conserved domains that could be important for its function. First, it has an AT-rich DNA interaction-type DNA-binding domain, which can specifically bind a DNA sequence known to be recognized by a SWI/SNF family-related complex at the beta-globin locus. Second, BAF250 stimulates glucocorticoid receptor-dependent transcriptional activation, and the stimulation is sharply reduced when the C-terminal region of BAF250 is deleted. This region of BAF250 is capable of interacting directly with the glucocorticoid receptor in vitro. Our data suggest that BAF250 confers specificity to the human BAF complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions.

MeSH Terms
Amino Acid Sequence Chromatin/metabolism Cloning, Molecular DNA Helicases DNA-Binding Proteins/genetics,metabolism Drosophila Proteins Globins/genetics Humans Molecular Sequence Data Multigene Family Nuclear Proteins Protein Structure, Tertiary Receptors, Glucocorticoid/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Species Specificity Transcription Factors Transcriptional Activation
Chemicals
ARID1A protein, human Chromatin DNA-Binding Proteins Drosophila Proteins Nuclear Proteins Receptors, Glucocorticoid SMARCA1 protein, human SMARCA2 protein, human Transcription Factors osa protein, Drosophila Globins SMARCA4 protein, human DNA Helicases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nie Z
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA.
Xue Y
Yang D
Zhou S
Deroo B J
Archer T K
Wang W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.23.8879-8888.2000
Published
2000-12-00
Pages
8879-88
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC86543
Subset
IM
Databases
GENBANK
AF231056
External Links
PubMed source DOI: 10.1128/MCB.20.23.8879-8888.2000 PMC Full Text View journal details
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