Home LiteratureArticle Details
PMID: 11073929 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

YsxC, a putative GTP-binding protein essential for growth of Bacillus subtilis 168.

Journal of bacteriology ·Vol. 182 ·No. 23 ·2000-12-00 ·Pages 6819-23

Prágai Z, Harwood CR

Abstract

YsxC is a member of a family of GTP-binding proteins carried by a diverse range of organisms from bacteria to yeasts, plants, and humans. To resolve the issue of whether ysxC of Bacillus subtilis is essential for growth, we attempted to construct mutants in which ysxC was either inactivated or placed under the control of an inducible promoter. Viable mutants were obtained only in the latter case, and these were inducer dependent, demonstrating unambiguously that ysxC is an essential gene.

MeSH Terms
Bacillus subtilis/genetics,growth & development,metabolism Bacterial Proteins/classification,genetics,metabolism GTP-Binding Proteins/classification,genetics,metabolism Gene Expression Mutagenesis
Chemicals
Bacterial Proteins GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prágai Z
Department of Microbiology and Immunology, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom.
Harwood C R
References (20)
20 references, click to expand
  1. Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis.
    J Bacteriol. 1995 Jun;177(11):3308-11 PMID: 7768831
  2. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  3. Bacillus subtilis lon protease prevents inappropriate transcription of genes under the control of the sporulation transcription factor sigma G.
    J Bacteriol. 1994 Nov;176(21):6528-37 PMID: 7961403
  4. Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):439-43 PMID: 6420789
  5. A perfectly symmetric lac operator binds the lac repressor very tightly.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6785-9 PMID: 6316325
  6. Obg, an essential GTP binding protein of Bacillus subtilis, is necessary for stress activation of transcription factor sigma(B).
    J Bacteriol. 1999 Aug;181(15):4653-60 PMID: 10419966
  7. A genome-based approach for the identification of essential bacterial genes.
    Nat Biotechnol. 1998 Sep;16(9):851-6 PMID: 9743119
  8. The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.
    Microbiology. 1996 Nov;142 ( Pt 11):3067-78 PMID: 8969504
  9. Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.
    J Bacteriol. 1994 Nov;176(21):6518-27 PMID: 7961402
  10. Principles governing amino acid composition of integral membrane proteins: application to topology prediction.
    J Mol Biol. 1998 Oct 23;283(2):489-506 PMID: 9769220
  11. The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.
    J Bacteriol. 1989 Mar;171(3):1362-71 PMID: 2537815
  12. Genomic microbiology: right on target?
    Nat Biotechnol. 1998 Sep;16(9):821-2 PMID: 9743110
  13. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
    Nature. 1999 Dec 16;402(6763):761-8 PMID: 10617197
  14. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.
    Nature. 1998 Mar 26;392(6674):353-8 PMID: 9537320
  15. The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
    Nature. 1997 Nov 20;390(6657):249-56 PMID: 9384377
  16. Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima.
    Nature. 1999 May 27;399(6734):323-9 PMID: 10360571
  17. A vector for systematic gene inactivation in Bacillus subtilis.
    Microbiology. 1998 Nov;144 ( Pt 11):3097-104 PMID: 9846745
  18. Proteins that interact with GTP during sporulation of Bacillus subtilis.
    J Bacteriol. 1989 Jun;171(6):2915-8 PMID: 2498282
  19. The European Bacillus subtilis genome sequencing project: current status and accessibility of the data from a new World Wide Web site.
    Microbiology. 1996 Nov;142 ( Pt 11):2987-91 PMID: 8969494
  20. Multiple sequence alignment with hierarchical clustering.
    Nucleic Acids Res. 1988 Nov 25;16(22):10881-90 PMID: 2849754
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.23.6819-6823.2000
Published
2000-12-00
Pages
6819-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC111427
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.23.6819-6823.2000 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2