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PMID: 11073913 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inorganic polyphosphate in Vibrio cholerae: genetic, biochemical, and physiologic features.

Journal of bacteriology ·Vol. 182 ·No. 23 ·2000-12-00 ·Pages 6687-93

Ogawa N, Tzeng CM, Fraley CD, Kornberg A

Abstract

Vibrio cholerae O1, biotype El Tor, accumulates inorganic polyphosphate (poly P) principally as large clusters of granules. Poly P kinase (PPK), the enzyme that synthesizes poly P from ATP, is encoded by the ppk gene, which has been cloned from V. cholerae, overexpressed, and knocked out by insertion-deletion mutagenesis. The predicted amino acid sequence of PPK is 701 residues (81.6 kDa), with 64% identity to that of Escherichia coli, which it resembles biochemically. As in E. coli, ppk is part of an operon with ppx, the gene that encodes exopolyphosphatase (PPX). However, unlike in E. coli, PPX activity was not detected in cell extracts of wild-type V. cholerae. The ppk null mutant of V. cholerae has diminished adaptation to high concentrations of calcium in the medium as well as motility and abiotic surface attachment.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial Diphosphotransferases/genetics,metabolism,physiology Genes, Bacterial Molecular Sequence Data Mutagenesis Phosphates/metabolism Phosphoprotein Phosphatases Polymers Vibrio cholerae/enzymology,genetics,metabolism
Chemicals
DNA, Bacterial Phosphates Polymers Diphosphotransferases nucleotide 3'-pyrophosphokinase-2',3'-cyclic monophosphokinase Phosphoprotein Phosphatases protein phosphatase 4
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ogawa N
Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA.
Tzeng C M
Fraley C D
Kornberg A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.23.6687-6693.2000
Published
2000-12-00
Pages
6687-93
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC111411
Subset
IM
Databases
GENBANK
AF083928
External Links
PubMed source DOI: 10.1128/JB.182.23.6687-6693.2000 PMC Full Text View journal details
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