Abstract
Escherichia coli transiently accumulates large amounts of inorganic polyphosphate (polyP), up to 20 mM in phosphate residues (Pi), in media deficient in both Pi and amino acids. This transient accumulation is preceded by the appearance of nucleotides ppGpp and pppGpp, generated in response to nutritional stresses. Mutants which lack PhoB, the response regulator of the phosphate regulon, do not accumulate polyP even though they develop wild-type levels of (p)ppGpp when subjected to amino acid starvation. When complemented with a phoB-containing plasmid, phoB mutants regain the ability to accumulate polyP. PolyP accumulation requires high levels of (p)ppGpp independent of whether they are generated by RelA (active during the stringent response) or SpoT (expressed during Pi starvation). Hence, accumulation of polyP requires a functional phoB gene and elevated levels of (p)ppGpp. A rapid assay of polyP depends on its adsorption to an anion-exchange disk on which it is hydrolyzed by a yeast exopolyphosphatase.
MeSH Terms
Bacterial Proteins/genetics,metabolism
DNA-Binding Proteins/metabolism
Escherichia coli/genetics,growth & development,metabolism
Genetic Complementation Test
Genotype
Guanosine Pentaphosphate/metabolism
Guanosine Tetraphosphate/metabolism
Kinetics
Models, Biological
Phosphates/metabolism
Polyphosphates/metabolism
Regulon
Chemicals
Bacterial Proteins
DNA-Binding Proteins
Phosphates
Polyphosphates
PhoB protein, Bacteria
Guanosine Tetraphosphate
Guanosine Pentaphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rao N N
Department of Biochemistry, Stanford University School of Medicine, California 94305-5307, USA.
Liu S
Kornberg A
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