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PMID: 2460731 Published · ppublish English Journal Article

Basal ppGpp level adjustment shown by new spoT mutants affect steady state growth rates and rrnA ribosomal promoter regulation in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 213 ·No. 2-3 ·1988-08-00 ·Pages 214-22

Sarubbi E, Rudd KE, Cashel M

Abstract

This work describes an approach towards analyzing the regulatory effects of variation of guanosine 3',5'-bispyrophosphate (ppGpp) basal levels in Escherichia coli during steady state growth. A series of strains was derived by mutating the spoT gene (which encodes the major cellular ppGppase) so as to obtain systematic increments in ppGpp basal levels. These strains differ genetically at the spoT locus and, in some cases, also at the relA locus because of the severity of spoT mutant alleles. Measurements of ppGpp revealed a ten-fold range of basal levels during growth on minimal medium. The empirical relationship between ppGpp concentration and growth rate is a simple linear inverse correlation. Tandem rrnA ribosomal RNA promoters, present on a multicopy plasmid, are shown to be differentially regulated over this range of basal levels. The upstream P1 promoter activity shows an inverse exponential relation to ppGpp concentration whereas the downstream P2 promoter is only weakly affected. We conclude that there are systematic regulatory consequences associated with small changes in ppGpp basal levels during steady state growth that probably are part of a continuum with more dramatic effects observed during the stringent response to amino acid deprivation.

MeSH Terms
Cell Division Culture Media Escherichia coli/genetics,growth & development,metabolism Gene Expression Regulation Guanine Nucleotides/metabolism Guanosine Tetraphosphate/metabolism Mutation Promoter Regions, Genetic RNA, Bacterial/genetics RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
Culture Media Guanine Nucleotides RNA, Bacterial RNA, Ribosomal Guanosine Tetraphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sarubbi E
Section of Molecular Regulation, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Rudd K E
Cashel M
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00339584
Published
1988-08-00
Pages
214-22
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00339584
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