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PMID: 11060033 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae.

The EMBO journal ·Vol. 19 ·No. 21 ·2000-11-01 ·Pages 5824-34

Chávez S, Beilharz T, Rondón AG, Erdjument-Bromage H, Tempst P, Svejstrup JQ, Lithgow T, Aguilera A

Abstract

Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of transcription-associated recombination, as they both affect transcription elongation and hyper-recombination in a concerted manner. Using a yeast strain that has the wild-type THO2 gene replaced by one encoding a His(6)-HA-tagged version, we have isolated an oligomeric complex containing four proteins: Tho2, Hpr1, Mft1 and a novel protein that we have named Thp2. We have reciprocally identified a complex containing Hpr1, Tho2 and Mft1 using anti-Mft1 antibodies in immunoprecipitation experiments. The protein complex is mainly nuclear; therefore, Tho2 and Hpr1 are physically associated. Like hpr1Delta and tho2Delta cells, mft1Delta and thp2Delta cells show mitotic hyper- recombination and impaired transcription elongation, in particular, through the bacterial lacZ sequence. Hyper-recombination conferred by mft1Delta and thp2Delta is only observed in DNA regions under transcription conditions. We propose that this protein complex acts as a functional unit connecting transcription elongation with the incidence of mitotic recombination.

MeSH Terms
Animals Base Sequence DNA Primers/genetics DNA-Binding Proteins Fungal Proteins/chemistry,genetics,metabolism Gene Expression Genes, Fungal Macromolecular Substances Mitosis Mutation Nuclear Proteins Phenotype Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins Subcellular Fractions/metabolism Temperature Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic
Chemicals
DNA Primers DNA-Binding Proteins Fungal Proteins HPR1 protein, S cerevisiae Macromolecular Substances Nuclear Proteins Saccharomyces cerevisiae Proteins THO2 protein, S cerevisiae Transcription Factors MFT1 protein, S cerevisiae
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chávez S
Departamento de Genética, Facultad de Biología, Avd. Reina Mercedes 6, Universidad de Sevilla, Sevilla 41012, Spain.
Beilharz T
Rondón A G
Erdjument-Bromage H
Tempst P
Svejstrup J Q
Lithgow T
Aguilera A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.21.5824
Published
2000-11-01
Pages
5824-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305808
Subset
IM
Grants
NCI NIH HHS · P30 CA008748 · United States
NCI NIH HHS · P30 CA08748 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.21.5824 PMC Full Text View journal details
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