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PMID: 2017143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene.

Molecular & general genetics : MGG ·Vol. 225 ·No. 3 ·1991-03-00 ·Pages 483-91

Garrett JM, Singh KK, Vonder Haar RA, Emr SD

Abstract

Mitochondrial targeting of an Atp2-LacZ fusion protein confers a respiration-defective phenotype on yeast cells. This effect has been utilized to select strains that grow on nonfermentable carbon sources, some of which have decreased levels of hybrid protein localized to the organelle. Many of the mutants obtained were also temperature-sensitive for growth on all media. The recessive mft (mitochondrial fusion targeting) mutants have been assigned to three complementation groups. MFT1 was cloned and sequenced: it encodes a 255 amino acid protein that is highly basic and has no predicted membrane-spanning domains or organelle-targeting sequences. The MFT1 gene is 91% identical to an open reading frame 3' of the SIR3 gene. Evidence is presented that these two closely related genes could represent a recent gene duplication.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal DNA-Binding Proteins Ethyl Methanesulfonate Fungal Proteins/genetics,metabolism Genes, Fungal Genetic Complementation Test Mitochondria/metabolism Molecular Sequence Data Mutation Open Reading Frames Oxygen Consumption/genetics Phenotype Recombinant Fusion Proteins/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Temperature
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins MFT1 protein, S cerevisiae Ethyl Methanesulfonate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garrett J M
Department of Biology, Hamilton College, Clinton, NY 13323.
Singh K K
Vonder Haar R A
Emr S D
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00261691
Published
1991-03-00
Pages
483-91
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 38253 · United States
Databases
GENBANK
M64566, S63796, S63798, S79268, S79270, S79277, S85222, S85224, X55360, X59005
External Links
PubMed source DOI: 10.1007/BF00261691
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