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PMID: 11050125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pax6 modulates the dorsoventral patterning of the mammalian telencephalon.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 20 ·No. 21 ·2000-11-01 ·Pages 8042-50

Stoykova A, Treichel D, Hallonet M, Gruss P

Abstract

The Pax6 gene encodes a transcription factor with a restricted expression in the ventricular zone of the pallium and subpallium. We tested whether the function of Pax6 is necessary for the correct patterning and morphogenesis of the vertebrate telencephalon. Homozygous embryos of the Pax6/Small eye mutant lack functional PAX6 protein because of a point mutation of the gene. In the mutant Small eye embryos we detected a ventralization of the molecular patterning of the telencephalon at two borders, the pallium/subpallium and the lateral/medial ganglionic eminence. The results indicate that Pax6 controls the lateral limit of the expression of Nkx2.1, Shh, and Lhx6 in the prechordal neural tube, the telencephalon. This finding is in agreement with previous studies and supports a model for a common genetic mechanism for modulation of the dorsoventral patterning of the prechordal and epichordal CNS. The pattern defects caused by the loss of Pax6 function result in multiple morphological abnormalities in the Small eye brain: dysgenesis of the piriform, insular, and lateral cortices, the claustrum-endopiriform nucleus, and a failure in the differentiation of a subpopulation of the cortical precursors. Together the results demonstrate that Pax6 has an essential role for the modulation of the dorsoventral patterning of the embryonic telencephalon, influencing thereby the forebrain morphogenesis.

MeSH Terms
Animals Antigens, Differentiation/biosynthesis Body Patterning/drug effects,physiology Cell Adhesion Molecules, Neuronal/biosynthesis Cell Differentiation/drug effects Cell Movement/drug effects Epithelial Cells/cytology,metabolism Epithelium/embryology Extracellular Matrix Proteins/biosynthesis Eye Proteins Homeodomain Proteins/metabolism,pharmacology Homozygote Immunohistochemistry In Situ Hybridization LIM-Homeodomain Proteins Mice Mice, Mutant Strains Nerve Tissue Proteins PAX6 Transcription Factor Paired Box Transcription Factors Reelin Protein Repressor Proteins Serine Endopeptidases Telencephalon/drug effects,embryology,metabolism Transcription Factors
Chemicals
Antigens, Differentiation Cell Adhesion Molecules, Neuronal Extracellular Matrix Proteins Eye Proteins Homeodomain Proteins LHX6 protein, mouse LIM-Homeodomain Proteins Nerve Tissue Proteins PAX6 Transcription Factor Paired Box Transcription Factors Pax6 protein, mouse Reelin Protein Repressor Proteins Transcription Factors Serine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stoykova A
Max-Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, 37077 Göttingen, Germany. astoyko@gwdg.de
Treichel D
Hallonet M
Gruss P
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Email
astoyko@gwdg.de
Published
2000-11-01
Pages
8042-50
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6772738
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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