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PMID: 11042145 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of mouse Iroquois homeobox genes in two clusters suggests a conserved regulation and function in vertebrate development.

Genome research ·Vol. 10 ·No. 10 ·2000-10-00 ·Pages 1453-62

Peters T, Dildrop R, Ausmeier K, Rüther U

Abstract

Iroquois proteins comprise a conserved family of homeodomain-containing transcription factors involved in patterning and regionalization of embryonic tissues in both vertebrates and invertebrates. Earlier studies identified four murine Iroquois (Irx) genes. Here we report the isolation of two additional members of the murine gene family, Irx5 and Irx6. Phylogenetic analysis of the Irx gene family revealed distinct clades for fly and vertebrate genes, and vertebrate members themselves were classified into three pairs of cognate genes. Mapping of the murine Irx genes identified two gene clusters located on mouse chromosomes 8 and 13, respectively. Each gene cluster is represented by three Irx genes whose relative positions within both clusters are strictly conserved. Combined results from phylogenetic, linkage, and physical mapping studies provide evidence for the evolution of two Irx gene clusters by duplication of a larger chromosomal region and dispersion to two chromosomal locations. The maintenance of two cognate Irx gene clusters during vertebrate evolution suggests that their genomic organization is important for the regulation, expression, and function of Irx genes during embryonic development.

MeSH Terms
Amino Acid Sequence Animals Chickens Conserved Sequence Drosophila Expressed Sequence Tags Gene Duplication Gene Expression Regulation, Developmental/genetics,physiology Genes, Homeobox/physiology Homeodomain Proteins/genetics,physiology Humans Mice Molecular Sequence Data Multigene Family Phylogeny Rats Transcription Factors/genetics,physiology Xenopus Zebrafish Zebrafish Proteins
Chemicals
Homeodomain Proteins IRX5 protein, human IRX6 protein, human Irx5 protein, mouse Irx6 protein, mouse Transcription Factors Zebrafish Proteins irx1a protein, zebrafish irx5a protein, zebrafish
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peters T
Entwicklungs- und Molekularbiologie der Tiere, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
Dildrop R
Ausmeier K
Rüther U
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.144100
Published
2000-10-00
Pages
1453-62
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC310936
Subset
IM
Databases
GENBANK
AJ271053, AJ271054, AJ271055
External Links
PubMed source DOI: 10.1101/gr.144100 PMC Full Text View journal details
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