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PMID: 10999605 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of 3' terminal adenylic acid residue on the uridylation of human small RNAs in vitro and in frog oocytes.

RNA (New York, N.Y.) ·Vol. 6 ·No. 9 ·2000-09-00 ·Pages 1277-88

Chen Y, Sinha K, Perumal K, Reddy R

Abstract

It is known that several small RNAs including human and Xenopus signal recognition particle (SRP) RNA, U2 small nuclear RNA (snRNA) and 7SK RNAs are posttranscriptionally adenylated, whereas U6 snRNA and ribosomal 5S RNA are posttranscriptionally uridylated on their 3' ends. In this study, we provide evidence that a small fraction of U6 snRNA and 5S ribosomal RNA molecules from human as well as Xenopus oocytes contain a single posttranscriptionally added adenylic acid residue on their 3' ends. These data show that U6 snRNA and 5S rRNAs are posttranscriptionally modified on their 3' ends by both uridylation and adenylation. Although the SRP RNA, 7SK RNA, 5S RNA, and U6 snRNA with the uridylic acid residue on their 3' ends were readily uridylated, all these RNAs with posttranscriptionally added adenylic acid residue on their 3' ends were not uridylated in vitro, or when U6 snRNA with 3' A(OH) was injected into Xenopus oocytes. These results show that the presence of a single posttranscriptionally added adenylic acid residue on the 3' end of SRP RNA, U6 snRNA, 5S rRNA, or 7SK RNA prevents 3' uridylation. These data also show that adenylation and uridylation are two competing processes that add nucleotides on the 3' end of some small RNAs and suggest that one of the functions of the 3' adenylation may be to negatively affect the 3' uridylation of small RNAs.

MeSH Terms
Adenosine Monophosphate/metabolism Alu Elements/physiology Animals HeLa Cells Humans Oocytes RNA Processing, Post-Transcriptional RNA, Ribosomal, 5S/metabolism RNA, Small Nuclear/chemistry,metabolism Signal Recognition Particle/genetics Transfection Uridine/metabolism Uridine Monophosphate/metabolism Xenopus
Chemicals
RNA, Ribosomal, 5S RNA, Small Nuclear Signal Recognition Particle Adenosine Monophosphate Uridine Monophosphate Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chen Y
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA.
Sinha K
Perumal K
Reddy R
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838200000285
Published
2000-09-00
Pages
1277-88
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370001
Subset
IM
Grants
NIGMS NIH HHS · GM 38320 · United States
NIGMS NIH HHS · GM 52901 · United States
External Links
PubMed source DOI: 10.1017/s1355838200000285 PMC Full Text View journal details
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