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PMID: 10889260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Auxin biosynthesis in maize kernels.

Plant physiology ·Vol. 123 ·No. 3 ·2000-07-00 ·Pages 1109-19

Glawischnig E, Tomas A, Eisenreich W, Spiteller P, Bacher A, Gierl A

Abstract

Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-(13)C(6)]glucose and [1, 2-(13)C(2)]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded.

MeSH Terms

Amino Acids/analysis Gas Chromatography-Mass Spectrometry Indoleacetic Acids/biosynthesis,metabolism Magnetic Resonance Spectroscopy Plant Growth Regulators/biosynthesis,metabolism Seeds/metabolism Tryptophan/metabolism Zea mays/metabolism

Chemicals

Amino Acids Indoleacetic Acids Plant Growth Regulators indoleacetic acid Tryptophan

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Glawischnig E

Lehrstuhl für Genetik, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany.

Tomas A

Eisenreich W

Spiteller P

Bacher A

Gierl A

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.123.3.1109

Published

2000-07-00

Pages

1109-19

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC59074

Subset

External Links

PubMed source DOI: 10.1104/pp.123.3.1109 PMC Full Text View journal details

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