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PMID: 1083675 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A characterization of human tonsillar lymphocytes after separation from other tonsillar cells in an isokinetic gradient of ficoll in tissue culture medium.

The American journal of pathology ·Vol. 83 ·No. 2 ·1976-05-00 ·Pages 341-58

Willson JK, Zaremba JL, Pitts AM, Pretlow TG

Abstract

Purified human tonsillar lymphocytes responded less to mitogentic stimulation than did unseparated tonsillar cells. Their response to mitogens was restored when they were combined with cells from all other gradient fractions. We interpret our data as evidence that the majority of tonsillar lymphocytes require the presence of more rapidly sedimenting cells for a maximum response to the tested mitogens. The purified tonsillar lymphoyctes were 47.0% lymphocytes that have detectable surface immunoglobulin and 29.9% lumphocytes that form rosettes with sheep red blood cells. The predominant cell surface immunoglobulin was IgM. Digestion of the tonsil with trypsin yielded tenfold more plasma cells, more vialbe cells, and a larger proportion of blasts, histiocytes, and binucleated cells than were obtained by mechanical dissociation of the tissue.

MeSH Terms
B-Lymphocytes/drug effects Cell Separation Culture Techniques Ficoll Humans Lectins/pharmacology Lymphocytes/cytology,drug effects Palatine Tonsil/cytology,drug effects T-Lymphocytes/drug effects Trypsin/pharmacology
Chemicals
Lectins Ficoll Trypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Willson J K
Zaremba J L
Pitts A M
Pretlow T G
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33 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1976-05-00
Pages
341-58
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC2032325
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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