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PMID: 4600344 Published · ppublish English Journal Article Review

Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens.

The Journal of experimental medicine ·Vol. 140 ·No. 1 ·1974-07-01 ·Pages 1-18

Greaves M, Janossy G, Doenhoff M

Abstract

Human lymphocytes from spleen and tonsils have been cultured with a variety of polyclonal mitogens. Cultures consisted of either unseparated T and B cells or alternatively purified T or B lymphocytes. The purity of the starting cell populations and the origin of activated lymphoblasts was analyzed with a panel of seven markers which discriminate between T and B cells. The selectivity of the lymphocyte responses was influenced by cell populations in a given culture, the mitogen used, and to a limited extent on culture conditions. Purified T lymphocytes from tonsil and spleen responded to phytohemagglutinin (PHA), pokeweed mitogen (PWM), and staphylococcal enterotoxin B (SEB). Purified B cells from spleen responded well to PWM, weakly to SEB and lipopolysaccharide, but not at all to PHA. Tonsil B cells responded weakly to PWM and SEB but not to PHA. Some B lymphocytes do respond to PHA in the presence of activated T cells. These results are discussed in relation to previously reported selective responses of human cells and parallel studies in animal species.

MeSH Terms
B-Lymphocytes/immunology Cell Division Cells, Cultured Clone Cells Enterotoxins/pharmacology Humans In Vitro Techniques Lectins/pharmacology Lipopolysaccharides/pharmacology Lymphocyte Activation Mitogens/pharmacology Palatine Tonsil/immunology Spleen/immunology Staphylococcus T-Lymphocytes/immunology
Chemicals
Enterotoxins Lectins Lipopolysaccharides Mitogens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Greaves M
Janossy G
Doenhoff M
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42 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.140.1.1
Published
1974-07-01
Pages
1-18
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139703
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.140.1.1 PMC Full Text View journal details
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