A new method for separating lymphocytes and granulocytes from human peripheral blood using programmed gradient sedimentation in an isokinetic gradient.

Immunology ·Vol. 24 ·No. 1 ·1973-01-00 ·Pages 85-92

Pretlow TG, Luberoff DE

Abstract

暂无摘要

MeSH Terms

Centrifugation, Density Gradient Culture Media Erythrocytes Humans Leukocyte Count Leukocytes Lymphocytes Methods Monocytes Polysaccharides

Chemicals

Culture Media Polysaccharides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pretlow T G

Luberoff D E

References (26)

26 references, click to expand

The isolation and selected properties of blood monocytes.
J Exp Med. 1966 Jan 1;123(1):145-60 PMID: 4285446
Ribonucleic acid metabolism in lymphocytes stimulated by phytohemagglutinin. II. Rapidly synthesized ribonucleic acid and te production of ribosomal ribonucleic acid.
J Biol Chem. 1968 Jan 10;243(1):34-43 PMID: 5635946
Ficoll flotation for the separation of blood leukocyte types.
Blood. 1968 Jan;31(1):66-73 PMID: 4966278
The resolution of mixtures of viable mammalian cells into homogeneous fractions by zonal centrifugation.
J Cell Biol. 1968 Feb;36(2):369-78 PMID: 5638886
Separation of biological particles by centrifugal elutriation.
Anal Biochem. 1968 Jun;23(3):369-77 PMID: 5658098
Purification of lymphocytes and platelets by gradient centrifugation.
J Lab Clin Med. 1968 Nov;72(5):842-8 PMID: 5697393
Cells involved in the immune response. X. The transfer of antibody-forming capacity to irradiated rabbits by antigen-reactive cells isolated from normal allogeneic rabbit bone marrow after passage through antigen-sensitized glass bead columns.
J Exp Med. 1969 Jul 1;130(1):141-63 PMID: 4893095
Rate zonal centrifugation in a Ficoll gradient.
Anal Biochem. 1969 May;29(2):230-7 PMID: 5792561
Separation of mammalian cells using programmed gradient sedimentation.
Exp Mol Pathol. 1969 Oct;11(2):139-52 PMID: 5344066
Isolation of leukemic blasts from peripheral blood by density gradient centrifugation.
J Lab Clin Med. 1970 Apr;75(4):703-10 PMID: 5444351
Rapid collection of human leukocytes or lymphocytes from peripheral blood.
Transplantation. 1969 Oct;8(4):535-8 PMID: 4986655
Cells involved in the immune response. XVII. The elution of antigen by antigen-reactive cells from antigen-sensitized glass bead columns. The high affinity of these cells for the glass bead-adsorbed antigen.
J Immunol. 1970 Jul;105(1):259-61 PMID: 4193276
Formation of granulocytes and macrophages in diffusion chamber cultures of mouse blood leucocytes.
Scand J Haematol. 1970;7(4):279-88 PMID: 5477542
The separation of different cell classes from lymphoid organs. IV. The separation of lymphocytes from phagocytes on glass bead columns, and its effect on subpopulations of lymphocytes and antibody-forming cells.
J Cell Biol. 1971 Mar;48(3):566-79 PMID: 4993515
Estimation of experimental conditions that permit cell separations by velocity sedimentation on isokinetic gradients of Ficoll in tissue culture medium.
Anal Biochem. 1971 May;41(1):248-55 PMID: 5578543
In vitro test for sensitivity of leukemic cells to L-asparaginase.
Cancer Res. 1971 May;31(5):523-6 PMID: 4932205
Cellular immunity to nephroblastoma.
Int J Cancer. 1971 Mar 15;7(2):277-84 PMID: 4325850
Separation of lymphocytes and macrophages from suspensions of guinea pig peritonitis exudate cells using programmed gradient sedimentation.
Am J Pathol. 1971 May;63(2):255-76 PMID: 5090640
Immunoblast formation by recirculating and non-recirculating rat lymphocytes cultured in diffusion chambers.
Scand J Haematol. 1971;8(1):32-43 PMID: 5566972
Deoxycytidine pathway in separated normal and leukemic leukocytes with effects of cell culture.
Blood. 1971 Sep;38(3):312-24 PMID: 5132149
A new method for separating peritoneal lymphocytes from macrophages.
Immunology. 1972 Jan;22(1):87-91 PMID: 5013912
Separation of beating cardiac myocytes from suspensions of heart cells.
Am J Pathol. 1972 May;67(2):215-26 PMID: 4336547
Separation of mast cells in successive stages of differentiation using programmed gradient sedimentation.
Am J Pathol. 1970 Dec;61(3):323-40 PMID: 4115156
Separation of ascites myeloma cells, lymphocytes and macrophages by zonal centrifugation on an isokinetic gradient.
Am J Pathol. 1972 Jul;68(1):163-82 PMID: 5080697
PLURIPOTENT STEM CELL FUNCTION OF THE MOUSE MARROW "LYMPHOCYTE".
Science. 1964 May 15;144(3620):866-8 PMID: 14149403
SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS.
Blood. 1964 Jun;23:811-28 PMID: 14161414

Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1973-01-00

Pages

85-92

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1422867

Subset

External Links

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A new method for separating lymphocytes and granulocytes from human peripheral blood using programmed gradient sedimentation in an isokinetic gradient.

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